BMRB Entry 31178

Name: NMR structure of the S. pombe Slr1 La motif RNA binding domain
Published: 2025-12-30

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PDB ID: 9cb2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31178
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the S. pombe Slr1 La motif RNA binding domain

Deposition date:

2024-06-18

Original release date:

2025-12-30

Authors:

Donaldson, L.

Citation:

Citation: Donaldson, L.. "NMR structure of the Tetrahymena Slr1 La Motif" .

Assembly members:

Assembly members:
entity_1, polymer, 113 residues, 13314.188 Da.

Natural source:

Natural source: Common Name: fission yeast Taxonomy ID: 284812 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDVQAFLTSQLEYYFSIENL SKDMFLRKHMDDEGYVPLAF LASFNRIKSFSTDLNLLHAA AKASDIIDVAEDLQSPMSIK VRRKETWSPWILPSESRLKF EMAKYLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	453
15N chemical shifts	106
1H chemical shifts	708

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 113 residues - 13314.188 Da.

1

MET

ASP

VAL

GLN

ALA

PHE

LEU

THR

SER

GLN

2

LEU

GLU

TYR

PHE

SER

ILE

GLU

ASN

LEU

3

SER

LYS

ASP

MET

PHE

LEU

ARG

LYS

HIS

MET

4

ASP

GLU

GLY

TYR

VAL

PRO

LEU

ALA

PHE

5

LEU

ALA

SER

PHE

ASN

ARG

ILE

LYS

SER

PHE

6

SER

THR

ASP

LEU

ASN

LEU

HIS

ALA

7

ALA

LYS

ALA

SER

ASP

ILE

ASP

VAL

ALA

8

GLU

ASP

LEU

GLN

SER

PRO

MET

SER

ILE

LYS

9

VAL

ARG

LYS

GLU

THR

TRP

SER

PRO

TRP

10

ILE

LEU

PRO

SER

GLU

SER

ARG

LEU

LYS

PHE

11

GLU

MET

ALA

LYS

TYR

LEU

GLU

HIS

12

HIS

Samples:

sample_1: Schizosaccharomyces pombe Slr1 La motif, [U-99% 13C; U-99% 15N], 0.8 mM; sodium phosphate 10 mM; sodium chloride 60 mM; sodium azide 0.05 % w/v

sample_conditions_1: ionic strength: 60 mM; pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

Rosetta, Baker laboratory - refinement

CYANA v3, Guntert, Mumenthaler and Wuthrich - structure calculation

CcpNmr Analysis, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker AVANCE III HD 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks