BMRB Entry 31078

Name: TCEI_III NMR Structure
Published: 2023-07-05

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PDB ID: 8sch
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31078
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

TCEI_III NMR Structure

Deposition date:

2023-04-05

Original release date:

2023-07-05

Authors:

Warden, M.; Mueller, G.; Hall, T.

Citation:

Citation: Warden, M.; DeRose, E.; Tamayo, J.; Mueller, G.; Gavis, E.; Hall, T.. "The translational repressor Glorund uses interchangeable RNA recognition domains to recognize Drosophila nanos" Nucleic Acids Res. 51, 8836-8849 (2023).
PubMed: 37427795

Assembly members:

Assembly members:
entity_1, polymer, 68 residues, 21838.988 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAGAAUCCAGCUCUGGAAGC GUUUAUAUAACAUGAAAUAU AUAUACGCAUUCCGAUCAAA GCUGGGUU

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	17

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 68 residues - 21838.988 Da.

1

G

A

G

A

U

C

A

G

2

C

U

C

U

G

A

G

C

3

G

U

A

U

A

U

A

4

C

A

U

G

A

U

A

U

5

A

U

A

U

A

C

G

C

A

U

6

U

C

G

A

U

C

A

7

G

C

U

G

U

Samples:

sample_1: TCEI_III 1 mM; sodium phosphate 10 mM

sample_2: TCEI_III, [U-100% 15N], 1 mM; sodium phosphate 10 mM; Pf1 phage, nat, 18 mg/mL

sample_conditions_1: ionic strength: 10 mM; pH: 6.8; pressure: 1 atm; temperature: 278 K

sample_conditions_2: ionic strength: 10 mM; pH: 6.8; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
1H 15N ARTSY	sample_2	anisotropic	sample_conditions_2
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	anisotropic	sample_conditions_2

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRViewJ, Johnson - chemical shift assignment, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

Agilent Direct Drive 800 MHz