BMRB Entry 31040

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31040
MolProbity Validation Chart

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Title:
Solution structure of the WH domain of MORF
Deposition date:
2022-08-19
Original release date:
2023-05-02
Authors:
Zhang, Y.; Kutateladze, T.
Citation:

Citation: Klein, B.; Zhang, Y.; Kutateladze, T.. "Structure of MORF-WH"  .

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, 9515.630 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GNDLRNVDWNKLLRRAIEGL EEPNGSSLKNIEKYLRSQSD LTSTTNNPAFQQRLRLGAKR AVNNGRLLKDGPQYRVNYGS LDGK

Data sets:
Data typeCount
13C chemical shifts188
15N chemical shifts91
1H chemical shifts459

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 84 residues - 9515.630 Da.

1   GLYASNASPLEUARGASNVALASPTRPASN
2   LYSLEULEUARGARGALAILEGLUGLYLEU
3   GLUGLUPROASNGLYSERSERLEULYSASN
4   ILEGLULYSTYRLEUARGSERGLNSERASP
5   LEUTHRSERTHRTHRASNASNPROALAPHE
6   GLNGLNARGLEUARGLEUGLYALALYSARG
7   ALAVALASNASNGLYARGLEULEULYSASP
8   GLYPROGLNTYRARGVALASNTYRGLYSER
9   LEUASPGLYLYS

Samples:

sample_1: MORF-WH, [U-13C; U-15N], 1.33 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

CcpNmr Analysis, CCPN - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Varian INOVA 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks