BMRB Entry 31016

Title:
Pis v 3.0101 vicilin leader sequence residues 56-115
Deposition date:
2022-04-29
Original release date:
2023-03-27
Authors:
Mueller, G.; Foo, A.; DeRose, E.
Citation:

Citation: Mueller, G.; Foo, A.; DeRose, E.. "Pis v 3.0101 vicilin leader sequence residues 56-115"  .

Assembly members:

Assembly members:
entity_1, polymer, 60 residues, 7309.122 Da.

Natural source:

Natural source:   Common Name: Pistacia vera   Taxonomy ID: 55513   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Pistacia vera

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts181
15N chemical shifts55
1H chemical shifts287

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 60 residues - 7309.122 Da.

1   GLYARGGLYASPGLUPHESERTHRHISGLU
2   PROGLYGLULYSARGLEUSERGLNCYSMET
3   LYSGLNCYSGLUARGGLNASPGLYGLYGLN
4   GLNLYSGLNLEUCYSARGPHEARGCYSGLN
5   GLULYSTYRLYSLYSGLUARGARGGLUHIS
6   SERTYRSERARGASPGLUGLULEUGLULEU

Samples:

sample_1: entity_1 mM; DSS 50 uM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

TALOS, Cornilescu, Delaglio and Bax - structure calculation

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMR spectrometers:

  • Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks