BMRB Entry 30979

Title:
Chickpea (Cicer arientinum) nodule-specific cysteine-rich peptide NCR13: Solution NMR structure of the isomer with C4:C10, C15:C30, and C23:C28 disulfide bonds
Deposition date:
2022-01-10
Original release date:
2022-01-28
Authors:
Buchko, G.; Zhou, M.; Shah, D.; Velivelli, S.
Citation:

Citation: Buchko, G.; Zhou, M.; Shah, D.; Velivelli, S.. "Chickpea (Cicer arientinum) nodule-specific cysteine-rich peptide NCR13: Solution NMR structure of the isomer with C4:C10, C15:C30, and C23:C28 disulfide bonds"  .

Assembly members:

Assembly members:
entity_1, polymer, 33 residues, 3741.605 Da.

Natural source:

Natural source:   Common Name: chickpea   Taxonomy ID: 3827   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Cicer arientinum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Komagataella pastoris

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ATKPCQSDKDCKKFACRKPK VPKCINGFCKCVR

Data sets:
Data typeCount
13C chemical shifts87
15N chemical shifts30
1H chemical shifts201

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 33 residues - 3741.605 Da.

1   ALATHRLYSPROCYSGLNSERASPLYSASP
2   CYSLYSLYSPHEALACYSARGLYSPROLYS
3   VALPROLYSCYSILEASNGLYPHECYSLYS
4   CYSVALARG

Samples:

sample_1: sodium acetate 20 ± 1 mM; sodium chloride 50 ± 1 mM; Def13_B2, [U-98% 15N], 1 ± 0.2 mM

sample_2: sodium acetate 20 ± 1 mM; sodium chloride 50 ± 1 mM; Def13_B2, [U-98% 15N], 1 ± 0.2 mM

sample_conditions_1: ionic strength: 70 mM; pH: 5.3; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
3D 1H-15N NOESYsample_2anisotropicsample_conditions_1
3D 1H-15N TOCSYsample_2anisotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1anisotropicsample_conditions_1
2D 1H-15N HSQCsample_1anisotropicsample_conditions_1
2D 1H-1H COSYsample_1anisotropicsample_conditions_1

Software:

Felix v75, Accelrys Software Inc. - processing

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMRFAM-SPARKY v3.135, bob - data analysis

PSVS v1.5, Bhattacharya and Montelione - data analysis

PdbStat, unknown - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker AVANCE III 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks