BMRB Entry 30956

Name: Solution structure of the zinc finger domain of murine MetAP1, complexed with ZNG N-terminal peptide
Published: 2022-05-26

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PDB ID: 7sek
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30956
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the zinc finger domain of murine MetAP1, complexed with ZNG N-terminal peptide

Deposition date:

2021-09-30

Original release date:

2022-05-26

Authors:

Edmonds, K.; Jordan, M.; Thalluri, K.; Wu, H.; Di Marchi, R.; Giedroc, D.

Citation:

Citation: Weiss, Andy; Murdoch, Caitlin; Edmonds, Katherine; Jordan, Matthew; Monteith, Andrew; Perera, Yasiru; Rodriguez Nassif, Aslin; Petoletti, Amber; Beavers, William; Munneke, Matthew; Drury, Sydney; Krystofiak, Evan; Thalluri, Kishore; Wu, Hongwei; Kruse, Angela; DiMarchi, Richard; Caprioli, Richard; Spraggins, Jeffrey; Chazin, Walter; Giedroc, David; Skaar, Eric. "Zn-regulated GTPase metalloprotein activator 1 modulates vertebrate zinc homeostasis" Cell 185, 2148-2163 (2022).
PubMed: 35584702

Assembly members:

Assembly members:
entity_1, polymer, 80 residues, 8956.125 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AEEEYAEDCPELVPIETKNQ EMAAVETRVCETDGCSSEAK LQCPTCIKLGIQGSYFCSQE CFKGSWATHKLLHKKAKDEK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list

Data type	Count
13C chemical shifts	347
15N chemical shifts	85
1H chemical shifts	547

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1
2	unit_2	2
3	unit_3	2

Entities:

Entity 1, unit_1 80 residues - 8956.125 Da.

1	ALA	GLU	GLU	GLU	TYR	ALA	GLU	ASP	CYS	PRO
2	GLU	LEU	VAL	PRO	ILE	GLU	THR	LYS	ASN	GLN
3	GLU	MET	ALA	ALA	VAL	GLU	THR	ARG	VAL	CYS
4	GLU	THR	ASP	GLY	CYS	SER	SER	GLU	ALA	LYS
5	LEU	GLN	CYS	PRO	THR	CYS	ILE	LYS	LEU	GLY
6	ILE	GLN	GLY	SER	TYR	PHE	CYS	SER	GLN	GLU
7	CYS	PHE	LYS	GLY	SER	TRP	ALA	THR	HIS	LYS
8	LEU	LEU	HIS	LYS	LYS	ALA	LYS	ASP	GLU	LYS

Entity 2, unit_2 - Zn - 65.409 Da.

1

ZN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3d Har(CC-TOCSY-CGCBCACO)NH	sample_1	isotropic	sample_conditions_1
2D hbCBcgcdceHE	sample_2	isotropic	sample_conditions_1
2D hbCBcgcdHD	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC long-range	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC CT	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC CT	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 30956

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	ALA	GLU	GLU	GLU	TYR	ALA	GLU	ASP	CYS	PRO
2	GLU	LEU	VAL	PRO	ILE	GLU	THR	LYS	ASN	GLN
3	GLU	MET	ALA	ALA	VAL	GLU	THR	ARG	VAL	CYS
4	GLU	THR	ASP	GLY	CYS	SER	SER	GLU	ALA	LYS
5	LEU	GLN	CYS	PRO	THR	CYS	ILE	LYS	LEU	GLY
6	ILE	GLN	GLY	SER	TYR	PHE	CYS	SER	GLN	GLU
7	CYS	PHE	LYS	GLY	SER	TRP	ALA	THR	HIS	LYS
8	LEU	LEU	HIS	LYS	LYS	ALA	LYS	ASP	GLU	LYS

1	ALA	GLU	GLU	GLU	TYR	ALA	GLU	ASP	CYS	PRO
2	GLU	LEU	VAL	PRO	ILE	GLU	THR	LYS	ASN	GLN
3	GLU	MET	ALA	ALA	VAL	GLU	THR	ARG	VAL	CYS
4	GLU	THR	ASP	GLY	CYS	SER	SER	GLU	ALA	LYS
5	LEU	GLN	CYS	PRO	THR	CYS	ILE	LYS	LEU	GLY
6	ILE	GLN	GLY	SER	TYR	PHE	CYS	SER	GLN	GLU
7	CYS	PHE	LYS	GLY	SER	TRP	ALA	THR	HIS	LYS
8	LEU	LEU	HIS	LYS	LYS	ALA	LYS	ASP	GLU	LYS

1	ALA	GLU	GLU	GLU	TYR	ALA	GLU	ASP	CYS	PRO
2	GLU	LEU	VAL	PRO	ILE	GLU	THR	LYS	ASN	GLN
3	GLU	MET	ALA	ALA	VAL	GLU	THR	ARG	VAL	CYS
4	GLU	THR	ASP	GLY	CYS	SER	SER	GLU	ALA	LYS
5	LEU	GLN	CYS	PRO	THR	CYS	ILE	LYS	LEU	GLY
6	ILE	GLN	GLY	SER	TYR	PHE	CYS	SER	GLN	GLU
7	CYS	PHE	LYS	GLY	SER	TRP	ALA	THR	HIS	LYS
8	LEU	LEU	HIS	LYS	LYS	ALA	LYS	ASP	GLU	LYS