BMRB Entry 30947

Name: NMR Solution Structure of hGal(1-12)KK, a solubility-tagged truncation of the human neuropeptide galanin
Published: 2022-05-17

Click here to enlarge.

PDB ID: 7s3q
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30947
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR Solution Structure of hGal(1-12)KK, a solubility-tagged truncation of the human neuropeptide galanin

Deposition date:

2021-09-08

Original release date:

2022-05-17

Authors:

Kraichely, K.; Hendy, C.; Parnham, S.; Giuliano, M.

Citation:

Citation: Kraichely, K.; Clinkscales, S.; Hendy, C.; Mendoza, E.; Parnham, S.; Giuliano, M.. "Minimal Increments of Hydrophobic Collapse within the N-Terminus of the Neuropeptide Galanin" Biochemistry 61, 1151-1166 (2022).
PubMed: 35622960

Assembly members:

Assembly members:
entity_1, polymer, 15 residues, 1507.757 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GWTLNSAGYLLGKKX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
1H chemical shifts	98

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 15 residues - 1507.757 Da.

1

GLY

TRP

THR

LEU

ASN

SER

ALA

GLY

TYR

LEU

2

LEU

GLY

LYS

NH2

Samples:

sample_1: hGal(2-12)KK 5 mM; sodium acetate, D3, 4.14 mM; acetic acid, D4, 5.86 mM

sample_conditions_1: ionic strength: 4.14 mM; pH: 4.6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H ROESY	sample_1	isotropic	sample_conditions_1
1D 1H acquisition	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al., Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

MestreLab (Mnova / MestReNova / MestReC), MestreLab (Mnova / MestReNova / MestReC) - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE II 600 MHz