BMRB Entry 30941

Name: I5R8 Mastoparano is a peptide synthetic, modified of Mastoparano (extracted from wasp venom)
Published: 2022-08-26

Click here to enlarge.

PDB ID: 7rul
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30941
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

I5R8 Mastoparano is a peptide synthetic, modified of Mastoparano (extracted from wasp venom)

Deposition date:

2021-08-17

Original release date:

2022-08-26

Authors:

Alves, E.; Santos, B.

Citation:

Citation: Alves, E.; Santos, B.. "Mastoparan I5R8, peptide antiviral derived of Mastoparan, venenon vesp." .

Assembly members:

Assembly members:
entity_1, polymer, 15 residues, 1609.119 Da.

Natural source:

Natural source: Common Name: Korean yellow-jacket wasp, Vespula flaviceps lewisii Taxonomy ID: 7452 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Vespula lewisii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSplPIGver2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: INLKILARLAKKILX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
13C chemical shifts	43
1H chemical shifts	133

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 15 residues - 1609.119 Da.

1

ILE

ASN

LEU

LYS

ILE

LEU

ALA

ARG

LEU

ALA

2

LYS

ILE

LEU

NH2

Samples:

sample_1: I5R8 2 mM; SDS-D25, [U-98% 2H], 100 mM; DSS, [U-98% 2H], 5 % v/v

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

TopSpin, Bruker Biospin - processing

CcpNmr Analysis, CCPN - data analysis

ARIA, Linge, O'Donoghue and Nilges - structure calculation

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

Bruker AVANCE 500 MHz