BMRB Entry 30853

Title:
Denv1 5'UTR Stem3
Deposition date:
2021-02-05
Original release date:
2022-02-04
Authors:
Sun, Y.; Varani, G.
Citation:

Citation: Sun, Y.; Varani, G.. "Structure of the Dengue Virus RNA Promoter"  RNA 28, 1210-1223 (2022).
PubMed: 35750488

Assembly members:

Assembly members:
entity_1, polymer, 28 residues, 9016.454 Da.

Natural source:

Natural source:   Common Name: Escherichia phage T7   Taxonomy ID: 10760   Superkingdom: Viruses   Kingdom: not available   Genus/species: Teseptimavirus Escherichia phage T7

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGCCGACAAGAACAGUUUCG AAUCGGCC

Data sets:
Data typeCount
1H chemical shifts119

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 28 residues - 9016.454 Da.

1   GGCCGACAAG
2   AACAGUUUCG
3   AAUCGGCC

Samples:

sample_1: Stem3 400 uM

sample_2: Stem3, [U-100% 13C; U-100% 15N], 400 uM

sample_3: Stem3, [U-100% 13C; U-100% 15N], 400 uM

sample_conditions_1: ionic strength: 40 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMRFAM-SPARKY, NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE II 600 MHz