BMRB Entry 30843

Name: Aspartimidylated Lasso Peptide Lihuanodin
Published: 2021-07-26

Click here to enlarge.

PDB ID: 7lcw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30843
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Aspartimidylated Lasso Peptide Lihuanodin

Deposition date:

2021-01-11

Original release date:

2021-07-26

Authors:

Link, A.; Cao, L.

Citation:

Citation: Cao, Li; Beiser, Moshe; Koos, Joseph; Orlova, Margarita; Elashal, Hader; Schroder, Hendrik; Link, A James. "Cellulonodin-2 and Lihuanodin: Lasso Peptides with an Aspartimide Post-Translational Modification" J. Am. Chem. Soc. 143, 11690-11702 (2021).
PubMed: 34283601

Assembly members:

Assembly members:
entity_1, polymer, 15 residues, 1753.780 Da.

Natural source:

Natural source: Common Name: Lihuaxuella thermophila Taxonomy ID: 1173111 Superkingdom: Bacteria Kingdom: not available Genus/species: Lihuaxuella thermophila

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSKYSDTADESSYRW

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
1H chemical shifts	84

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 15 residues - 1753.780 Da.

1

GLY

SER

LYS

TYR

SER

ASP

THR

ALA

ASP

GLU

2

SER

TYR

ARG

TRP

Samples:

sample_1: lihuanodin 1.6 mM

sample_conditions_1: ionic strength: 0 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D TOCSY	sample_1	isotropic	sample_conditions_1
2D NOESY	sample_1	isotropic	sample_conditions_1

Software:

MestreLab (Mnova / MestReNova / MestReC), MestreLab Research - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

NMR spectrometers:

Bruker AVANCE III 800 MHz