BMRB Entry 30843

Title:
Aspartimidylated Lasso Peptide Lihuanodin
Deposition date:
2021-01-11
Original release date:
2021-07-26
Authors:
Link, A.; Cao, L.
Citation:

Citation: Cao, Li; Beiser, Moshe; Koos, Joseph; Orlova, Margarita; Elashal, Hader; Schroder, Hendrik; Link, A James. "Cellulonodin-2 and Lihuanodin: Lasso Peptides with an Aspartimide Post-Translational Modification"  J. Am. Chem. Soc. 143, 11690-11702 (2021).
PubMed: 34283601

Assembly members:

Assembly members:
entity_1, polymer, 15 residues, 1753.780 Da.

Natural source:

Natural source:   Common Name: Lihuaxuella thermophila   Taxonomy ID: 1173111   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Lihuaxuella thermophila

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSKYSDTADESSYRW

Data sets:
Data typeCount
1H chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 15 residues - 1753.780 Da.

1   GLYSERLYSTYRSERASPTHRALAASPGLU
2   SERSERTYRARGTRP

Samples:

sample_1: lihuanodin 1.6 mM

sample_conditions_1: ionic strength: 0 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYsample_1isotropicsample_conditions_1
2D NOESYsample_1isotropicsample_conditions_1

Software:

MestreLab (Mnova / MestReNova / MestReC), MestreLab Research - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

NMR spectrometers:

  • Bruker AVANCE III 800 MHz