BMRB Entry 30830

Name: Solution NMR structure of the cyclic plant protein PDP-23 in DPC micelles
Published: 2021-01-25

Click here to enlarge.

PDB ID: 7l55
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30830
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution NMR structure of the cyclic plant protein PDP-23 in DPC micelles

Deposition date:

2020-12-21

Original release date:

2021-01-25

Authors:

Payne, C.; Rosengren, K.

Citation:

Citation: Payne, C.; Franke, B.; Fisher, M.; Hajiaghaalipour, F.; McAleese, C.; Song, A.; Eliasson, C.; Zhang, J.; Jayasena, A.; Vadlamani, G.; Clark, R.; Minchin, R.; Mylne, J.; Rosengren, K.. "A chameleonic macrocyclic peptide with drug delivery applications" Chem. Sci. 12, 6670-6683 (2021).
PubMed: 34040741

Assembly members:

Assembly members:
entity_1, polymer, 28 residues, 3135.512 Da.

Natural source:

Natural source: Common Name: garden zinnia Taxonomy ID: 34245 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Zinnia elegans

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GFCWHHSCVPSGTCADFPWP LGHQCFPD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	37
1H chemical shifts	170

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 28 residues - 3135.512 Da.

1

GLY

PHE

CYS

TRP

HIS

SER

CYS

VAL

PRO

2

SER

GLY

THR

CYS

ALA

ASP

PHE

PRO

TRP

PRO

3

LEU

GLY

HIS

GLN

CYS

PHE

PRO

ASP

Samples:

sample_1: PDP-23 2 mg/mL; DPC 12 mM

sample_conditions_1: ionic strength: 0 mM; pH: 5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0 mM; pH: 5; pressure: 1 atm; temperature: 288 K

sample_conditions_3: ionic strength: 0 mM; pH: 5; pressure: 1 atm; temperature: 303 K

sample_conditions_4: ionic strength: 0 mM; pH: 5; pressure: 1 atm; temperature: 293 K

sample_conditions_5: ionic strength: 0 mM; pH: 5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_2
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_4
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_5
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_3

Software:

TopSpin, Bruker Biospin - data analysis, processing

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

NMR spectrometers:

Bruker AVANCE III 700 MHz