BMRB Entry 30797

Title:
Dengue 5'UTR SLA
Deposition date:
2020-09-15
Original release date:
2021-09-22
Authors:
Sun, Y.
Citation:

Citation: Sun, Y.. "Solution NMR Structure of Denv1 5'UTR SLA region"  .

Assembly members:

Assembly members:
entity_1, polymer, 36 residues, 11521.802 Da.

Natural source:

Natural source:   Common Name: Escherichia phage T7   Taxonomy ID: 10760   Superkingdom: Viruses   Kingdom: not available   Genus/species: Teseptimavirus Escherichia virus T7

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGAGUGGUUAGUCUACGUUU CGACGUAGUUCUAACC

Data sets:
Data typeCount
1H chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 36 residues - 11521.802 Da.

1   GGAGUGGUUA
2   GUCUACGUUU
3   CGACGUAGUU
4   CUAACC

Samples:

sample_1: DenvSLA 200 uM; potassium phosphate 10 mM; potassium chloride 10 mM

sample_2: DenvSLA, [U-100% 2H], 200 uM; potassium phosphate 10 mM; potassium chloride 10 mM

sample_conditions_1: ionic strength: 40 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMRFAM-SPARKY, Lee W, Tonelli M, Markley JL - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE II 600 MHz
  • Bruker AVANCE II 500 MHz