BMRB Entry 30765
Click here to enlarge.
PDB ID: 6xfl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR30765
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Majewski, D.; Okon, M.; Heinkel, F.; Robb, C.; Vuckovic, M.; McIntosh, L.; Strynadka, N.. "Characterization of the pilotin-secretin complex from the Salmonella enterica type III secretion system using hybrid structural methods" Structure 29, 125-138 (2021).
PubMed: 32877645
Assembly members:
entity_1, polymer, 82 residues, 9453.652 Da.
entity_2, polymer, 47 residues, 5248.818 Da.
Natural source: Common Name: Salmonella enterica Taxonomy ID: 99287 Superkingdom: Bacteria Kingdom: not available Genus/species: Salmonella enterica
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)
Entity Sequences (FASTA):
entity_1: GSHMDNSASKNSAISSSIFC
EKYKQTKEQALTFFQEHPQY
MRSKEDEEQLMTEFKKVLLE
PGSKNLSIYQTLLAAHERLQ
AL
entity_2: GSHMDPLTPDASESVNNILK
QSGAWSGDDKLQKWVRVYLD
RGQEAIK
| Data type | Count |
| 13C chemical shifts | 551 |
| 15N chemical shifts | 134 |
| 1H chemical shifts | 876 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
| 2 | unit_2 | 2 |
Entities:
Entity 1, unit_1 82 residues - 9453.652 Da.
| 1 | GLY | SER | HIS | MET | ASP | ASN | SER | ALA | SER | LYS | ||||
| 2 | ASN | SER | ALA | ILE | SER | SER | SER | ILE | PHE | CYS | ||||
| 3 | GLU | LYS | TYR | LYS | GLN | THR | LYS | GLU | GLN | ALA | ||||
| 4 | LEU | THR | PHE | PHE | GLN | GLU | HIS | PRO | GLN | TYR | ||||
| 5 | MET | ARG | SER | LYS | GLU | ASP | GLU | GLU | GLN | LEU | ||||
| 6 | MET | THR | GLU | PHE | LYS | LYS | VAL | LEU | LEU | GLU | ||||
| 7 | PRO | GLY | SER | LYS | ASN | LEU | SER | ILE | TYR | GLN | ||||
| 8 | THR | LEU | LEU | ALA | ALA | HIS | GLU | ARG | LEU | GLN | ||||
| 9 | ALA | LEU |
Entity 2, unit_2 47 residues - 5248.818 Da.
| 1 | GLY | SER | HIS | MET | ASP | PRO | LEU | THR | PRO | ASP | ||||
| 2 | ALA | SER | GLU | SER | VAL | ASN | ASN | ILE | LEU | LYS | ||||
| 3 | GLN | SER | GLY | ALA | TRP | SER | GLY | ASP | ASP | LYS | ||||
| 4 | LEU | GLN | LYS | TRP | VAL | ARG | VAL | TYR | LEU | ASP | ||||
| 5 | ARG | GLY | GLN | GLU | ALA | ILE | LYS |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks