BMRB Entry 30760

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30760
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
NMR structure of Ost4 in DPC micelles
Deposition date:
2020-06-09
Original release date:
2021-02-04
Authors:
Chaudhary, B.
Citation:

Citation: Chaudhary, Bharat; Zoetewey, David; McCullagh, Martin; Mohanty, Smita. "NMR and MD simulations reveal the impact of the V23D mutation on the function of yeast oligosaccharyltransferase subunit Ost4"  Glycobiology 31, 838-850 (2021).
PubMed: 33442744

Assembly members:

Assembly members:
entity_1, polymer, 45 residues, 5215.042 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 307796   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MISDEQLNSLAITFGIVMMT LIVIYHAVDSTMSPKNRLEH HHHHH

Data sets:
Data typeCount
13C chemical shifts109
15N chemical shifts40
1H chemical shifts257

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 45 residues - 5215.042 Da.

1   METILESERASPGLUGLNLEUASNSERLEU
2   ALAILETHRPHEGLYILEVALMETMETTHR
3   LEUILEVALILETYRHISALAVALASPSER
4   THRMETSERPROLYSASNARGLEUGLUHIS
5   HISHISHISHISHIS

Samples:

sample_1: Yeast Oligosaccharyltransferase subunit Ost4, [U-13C; U-15N], 400 uM; DPC micelle 100 mM

sample_conditions_1: ionic strength: 400 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRFAM-SPARKY, Lee, W., Tonelli, M., and Markley, J. L - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker AVANCE 800 MHz
  • Varian INOVA 900 MHz
  • Bruker AVANCE 700 MHz
  • Bruker AVANCE 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks