BMRB Entry 30754

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30754
MolProbity Validation Chart

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Title:
NMR solution structure of Asterix/Gtsf1 from mouse (CHHC zinc finger domains)
Deposition date:
2020-05-22
Original release date:
2021-02-20
Authors:
Ipsaro, J.; O'Brien, P.; Bhattacharya, S.; Palmer III, A.; Joshua-Tor, L.
Citation:

Citation: Ipsaro, J.; O'Brien, P.; Bhattacharya, S.; Palmer III, A.; Joshua-Tor, L.. "Asterix/Gtsf1 links tRNAs and piRNA silencing of retrotransposons"  Cell Rep. 34, 108914-108914 (2021).
PubMed: 33789107

Assembly members:

Assembly members:
entity_1, polymer, 121 residues, 14217.607 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Spodoptera frugiperda   Vector: pFL

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEDTYIDSLDPEKLLQCPYD KNHQIRASRFPYHLIKCRKN HPDVANKLATCPFNARHQVP RAEISHHISSCDDKSSIEQD VVNQTRNLGQETLAESTWQS PPSDEDWDKDLWEQTENLYF Q

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts80
1H chemical shifts485

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22
3unit_32

Entities:

Entity 1, unit_1 121 residues - 14217.607 Da.

1   METGLUASPTHRTYRILEASPSERLEUASP
2   PROGLULYSLEULEUGLNCYSPROTYRASP
3   LYSASNHISGLNILEARGALASERARGPHE
4   PROTYRHISLEUILELYSCYSARGLYSASN
5   HISPROASPVALALAASNLYSLEUALATHR
6   CYSPROPHEASNALAARGHISGLNVALPRO
7   ARGALAGLUILESERHISHISILESERSER
8   CYSASPASPLYSSERSERILEGLUGLNASP
9   VALVALASNGLNTHRARGASNLEUGLYGLN
10   GLUTHRLEUALAGLUSERTHRTRPGLNSER
11   PROPROSERASPGLUASPTRPASPLYSASP
12   LEUTRPGLUGLNTHRGLUASNLEUTYRPHE
13   GLN

Entity 2, unit_2 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Gtsf1, [U-15N], 0.5 mM; NaCl 200 mM

sample_2: Gtsf1, [U-13C; U-15N], 0.8 mM; NaCl 200 mM

sample_3: Gtsf1, [U-13C; U-15N], 0.8 mM; NaCl 200 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D (H)C(CCO)NHsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_3isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer, Bax - processing

Sparky, Lee, Tonelli, Markley - chemical shift assignment, peak picking

ARIA v2.3, Linge, Habeck, Rieping, Nilges - refinement, structure calculation

CNS v1.2, Brunger - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker AVANCE III 700 MHz
  • Bruker AVANCE 800 MHz
  • Bruker AVANCE 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks