BMRB Entry 30723

Name: Cap3G-TAR-F1
Published: 2020-04-27

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PDB ID: 6vu1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30723
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Cap3G-TAR-F1

Deposition date:

2020-02-14

Original release date:

2020-04-27

Authors:

Summers, M.; Brown, J.

Citation:

Citation: Brown, Joshua; Kharytonchyk, Siarhei; Chaudry, Issac; Iyer, Aishwarya; Carter, Hannah; Becker, Ghazal; Desai, Yash; Glang, Lindsay; Choi, Seung; Singh, Karndeep; Lopresti, Michael; Orellana, Matthew; Rodriguez, Tatiana; Oboh, Ubiomo; Hijji, Jana; Ghinger, Frances Grace; Stewart, Kailan; Francis, Dillion; Edwards, Bryce; Chen, Patrick; Case, David; Telesnitsky, Alice; Summers, Michael. "Structural basis for transcriptional start site control of HIV-1 RNA fate" Science 368, 413-417 (2020).
PubMed: 32327595

Assembly members:

Assembly members:
entity_1, polymer, 34 residues, 11041.558 Da.

Natural source:

Natural source: Common Name: HIV-1 Taxonomy ID: 11676 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XGGGUCUCUCUUGUCGAGAG GCAAGGAACCCACU

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	160

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 34 residues - 11041.558 Da.

1

8NK

G

U

C

U

C

U

C

2

U

G

U

C

G

A

G

A

G

3

G

C

A

G

A

C

4

C

A

C

U

Samples:

sample_1: Cap3G-TAR-F1, Fully protonated, 150 uM; KH2PO4, Deuterated, 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.4 pD; pressure: 1 atm; temperature: 308 K

sample_conditions_2: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_2

Software:

Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMRFx, Johnson, One Moon Scientific - processing

NMR spectrometers:

Bruker AVANCE 600 MHz