BMRB Entry 30698

Name: Solution Structure of the Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MH5
Published: 2020-05-15

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PDB ID: 6va2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30698
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MH5

Deposition date:

2019-12-16

Original release date:

2020-05-15

Authors:

Chen, J.; Fountain, M.; Disney, M.

Citation:

Citation: Chen, Jonathan; Zhang, Peiyuan; Abe, Masahito; Aikawa, Haruo; Zhang, Liying; Frank, Alexander; Zembryski, Timothy; Hubbs, Christopher; Park, HaJeung; Withka, Jane; Steppan, Claire; Rogers, Lucy; Cabral, Shawn; Pettersson, Martin; Wager, Travis; Fountain, Matthew; Rumbaugh, Gavin; Childs-Disney, Jessica; Disney, Matthew. "Design, Optimization, and Study of Small Molecules That Target Tau Pre-mRNA and Affect Splicing" J. Am. Chem. Soc. 142, 8706-8727 (2020).
PubMed: 32364710

Assembly members:

Assembly members:
entity_1, polymer, 11 residues, 3538.154 Da.
entity_2, polymer, 10 residues, 3175.964 Da.
entity_QSV, non-polymer, 408.523 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: CCGGCAGUGUG
entity_2: CACACGUCGG

Data sets:

assigned_chemical_shifts

Data type	Count
1H chemical shifts	156

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2
3	entity_3	3

Entities:

Entity 1, entity_1 11 residues - 3538.154 Da.

1

C

G

C

A

G

U

G

U

2

G

Entity 2, entity_2 10 residues - 3175.964 Da.

1

C

A

C

A

C

G

U

C

G

Entity 3, entity_3 - C20 H24 N8 S - 408.523 Da.

1

QSV

Samples:

sample_1: WT Tau RNA 0.7 mM; WT Tau RNA 0.7 mM; MH5 0.7 mM; KH2PO4/K2HPO4 10 mM; EDTA 0.05 mM

sample_2: WT Tau RNA 0.7 mM; WT Tau RNA 0.7 mM; MH5 0.7 mM; KH2PO4/K2HPO4 10 mM; EDTA 0.05 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 281.5 K

Experiments:

Name	Sample	Sample state	Sample conditions
D2O 2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
H2O 2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_2
D2O 2D DQF-COSY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TopSpin, Bruker Biospin - processing

Sparky, Goddard - chemical shift assignment

Amber v14, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollman - refinement, structure calculation

NMR spectrometers:

Bruker AVANCE III 700 MHz