BMRB Entry 30658

Name: Solution structure of paxillin LIM4
Published: 2019-10-16

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PDB ID: 6u4m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30658
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of paxillin LIM4

Deposition date:

2019-08-26

Original release date:

2019-10-16

Authors:

Zhu, L.; Qin, J.

Citation:

Citation: Zhu, Liang; Liu, Huan; Lu, Fan; Yang, Jun; Byzova, Tatiana; Qin, Jun. "Structural Basis of Paxillin Recruitment by Kindlin-2 in Regulating Cell Adhesion" Structure 27, 1686-1697 (2019).
PubMed: 31590942

Assembly members:

Assembly members:
entity_1, polymer, 72 residues, 8309.760 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3) Vector: pGST-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMDPEFYHERRGSLCSGCQ KPITGRCITAMAKKFHPEHF VCAFCLKQLNKGTFKEQNDK PYCQNCFLKLFC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	276
15N chemical shifts	72
1H chemical shifts	421

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2, 1	2
3	entity_2, 2	2

Entities:

Entity 1, entity_1 72 residues - 8309.760 Da.

1

GLY

ALA

MET

ASP

PRO

GLU

PHE

TYR

HIS

GLU

2

ARG

GLY

SER

LEU

CYS

SER

GLY

CYS

GLN

3

LYS

PRO

ILE

THR

GLY

ARG

CYS

ILE

THR

ALA

4

MET

ALA

LYS

PHE

HIS

PRO

GLU

HIS

PHE

5

VAL

CYS

ALA

PHE

CYS

LEU

LYS

GLN

LEU

ASN

6

LYS

GLY

THR

PHE

LYS

GLU

GLN

ASN

ASP

LYS

7

PRO

TYR

CYS

GLN

ASN

CYS

PHE

LEU

LYS

LEU

8

PHE

CYS

Entity 2, entity_2, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Paxillin LIM4, [U-13C; U-15N], 1.45 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 .; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CCCONH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 15N/13C-edited NOESY	sample_1	isotropic	sample_conditions_1
3D HCCCONH	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

Sparky, Goddard - chemical shift assignment

PIPP, Garrett - peak picking

PASA, Xu, Wang, Yang, Vaynberg, and Qin - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

Bruker AVANCE III 850 MHz
Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks