BMRB Entry 30653

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30653
MolProbity Validation Chart

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Title:
Human CstF-64 RRM mutant - D50A
Deposition date:
2019-08-12
Original release date:
2020-08-14
Authors:
Latham, M.; Masoumzadeh, E.
Citation:

Citation: Grozdanov, Petar; Masoumzadeh, Elahe; Kalscheuer, Vera; Bienvenu, Thierry; Billuart, Pierre; Delrue, Marie-Ange; Latham, Michael; MacDonald, Clinton. "A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3' end processing is associated with intellectual disability in humans"  Nucleic Acids Res. 48, 9804-9821 (2020).
PubMed: 32816001

Assembly members:

Assembly members:
entity_1, polymer, 110 residues, 12117.579 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAGLTVRDPAVDRSLRSVFV GNIPYEATEEQLKDIFSEVG PVVSFRLVYARETGKPKGYG FCEYQDQETALSAMRNLNGR EFSGRALRVDNAASEKNKEE LKSLGTGAPV

Data sets:
Data typeCount
13C chemical shifts223
15N chemical shifts100
1H chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 110 residues - 12117.579 Da.

1   METALAGLYLEUTHRVALARGASPPROALA
2   VALASPARGSERLEUARGSERVALPHEVAL
3   GLYASNILEPROTYRGLUALATHRGLUGLU
4   GLNLEULYSASPILEPHESERGLUVALGLY
5   PROVALVALSERPHEARGLEUVALTYRALA
6   ARGGLUTHRGLYLYSPROLYSGLYTYRGLY
7   PHECYSGLUTYRGLNASPGLNGLUTHRALA
8   LEUSERALAMETARGASNLEUASNGLYARG
9   GLUPHESERGLYARGALALEUARGVALASP
10   ASNALAALASERGLULYSASNLYSGLUGLU
11   LEULYSSERLEUGLYTHRGLYALAPROVAL

Samples:

sample_1: CstF-64 RNA recognition motif mutant, [U-100% 13C; U-100% 15N], 1.2 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, peak picking

CS-ROSETTA, Shen, Vernon, Baker and Bax - refinement, structure calculation

NMR spectrometers:

  • Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks