BMRB Entry 30644

Name: Backbone-modified variant of zinc finger 2 from the transcription factor Sp1 DNA binding domain: altered helix, loop, turn, and sheet
Published: 2020-06-19

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PDB ID: 6pv3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30644
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone-modified variant of zinc finger 2 from the transcription factor Sp1 DNA binding domain: altered helix, loop, turn, and sheet

Deposition date:

2019-07-19

Original release date:

2020-06-19

Authors:

Rao, S.; Horne, W.

Citation:

Citation: Rao, S.; Horne, W.. "Proteomimetic Zinc Finger Domains with Modified Metal-binding beta-Turns" Pept. Sci. (Hoboken) 112, e24177-e24177 (2020).
PubMed: 33733039

Assembly members:

Assembly members:
entity_1, polymer, 31 residues, 3649.238 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PFXCTWXGCGKXFTRSXELQ XHKXTHXGEKX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	196

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	ZINC ION	2

Entities:

Entity 1, entity_1 31 residues - 3649.238 Da.

1

PRO

PHE

9KK

CYS

THR

TRP

AIB

GLY

CYS

GLY

2

LYS

MMO

PHE

THR

ARG

SER

B3D

GLU

LEU

GLN

3

HMR

HIS

LYS

HMR

THR

HIS

B3T

GLY

GLU

LYS

4

NH2

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: entity_1 1.2 mM; TRIS, [U-2H], 10 mM; DSS 0.05 mM; zinc chloride 1.8 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6 pH*; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TopSpin, Bruker Biospin - processing

Sparky, Goddard - data analysis

ARIA, Linge, O'Donoghue and Nilges - refinement, structure calculation

NMR spectrometers:

Bruker Avance 700 MHz