BMRB Entry 30643

Name: Backbone-modified variant of zinc finger 2 from the transcription factor Sp1 DNA binding domain: Orn in the metal-binding turn
Published: 2020-06-19

Click here to enlarge.

PDB ID: 6pv2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30643
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone-modified variant of zinc finger 2 from the transcription factor Sp1 DNA binding domain: Orn in the metal-binding turn

Deposition date:

2019-07-19

Original release date:

2020-06-19

Authors:

Rao, S.; Horne, W.

Citation:

Citation: Rao, S.; Horne, W.. "Proteomimetic Zinc Finger Domains with Modified Metal-binding beta-Turns" Pept. Sci. (Hoboken) 112, e24177-e24177 (2020).
PubMed: 33733039

Assembly members:

Assembly members:
entity_1, polymer, 31 residues, 3697.285 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RPFXCTWXCGKRFTRSDELQ RHKRTHTGEKX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	206

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	ZINC ION	2

Entities:

Entity 1, entity_1 31 residues - 3697.285 Da.

1

ARG

PRO

PHE

NLE

CYS

THR

TRP

ORN

CYS

GLY

2

LYS

ARG

PHE

THR

ARG

SER

ASP

GLU

LEU

GLN

3

ARG

HIS

LYS

ARG

THR

HIS

THR

GLY

GLU

LYS

4

NH2

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: entity_1 1.2 mM; TRIS, [U-2H], 10 mM; DSS 0.05 mM; zinc chloride 1.8 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6 pH*; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TopSpin, Bruker Biospin - processing

Sparky, Goddard - data analysis

ARIA, Linge, O'Donoghue and Nilges - refinement, structure calculation

NMR spectrometers:

Bruker Avance 700 MHz