BMRB Entry 30637

Name: Structural Basis for Client Recognition and Activity of Hsp40 Chaperones
Published: 2019-09-11

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PDB ID: 6prq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30637
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural Basis for Client Recognition and Activity of Hsp40 Chaperones

Deposition date:

2019-07-10

Original release date:

2019-09-11

Authors:

Jiang, Y.; Rossi, P.; Kalodimos, C.

Citation:

Citation: Jiang, Y.; Rossi, P.; Kalodimos, C.. "Structural basis for client recognition and activity of Hsp40 chaperones" Science 365, 1313-1319 (2019).
PubMed: 31604242

Assembly members:

Assembly members:
entity_1, polymer, 161 residues, 16625.180 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 83333 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AALVAHVTSGSGGSGGSGGS GRDLRAELPLTLEEAFHGGE RVVEVAGRRVSVRIPPGVRE GSVIRVPGMGGQGNPPGDLL LVVRLLPHPVFRLEGQDLYA TLDVPAPIAVVGGKVRAMTL EGPVEVAVPPRTQAGRKLRL KGKGFPGPAGRGDLYLEVRI T

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	311
15N chemical shifts	132
1H chemical shifts	426

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 161 residues - 16625.180 Da.

1

ALA

LEU

VAL

ALA

HIS

VAL

THR

SER

GLY

2

SER

GLY

SER

GLY

SER

GLY

SER

3

GLY

ARG

ASP

LEU

ARG

ALA

GLU

LEU

PRO

LEU

4

THR

LEU

GLU

ALA

PHE

HIS

GLY

GLU

5

ARG

VAL

GLU

VAL

ALA

GLY

ARG

VAL

6

SER

VAL

ARG

ILE

PRO

GLY

VAL

ARG

GLU

7

GLY

SER

VAL

ILE

ARG

VAL

PRO

GLY

MET

GLY

8

GLY

GLN

GLY

ASN

PRO

GLY

ASP

LEU

9

LEU

VAL

ARG

LEU

PRO

HIS

PRO

VAL

10

PHE

ARG

LEU

GLU

GLY

GLN

ASP

LEU

TYR

ALA

11

THR

LEU

ASP

VAL

PRO

ALA

PRO

ILE

ALA

VAL

12

VAL

GLY

LYS

VAL

ARG

ALA

MET

THR

LEU

13

GLU

GLY

PRO

VAL

GLU

VAL

ALA

VAL

PRO

14

ARG

THR

GLN

ALA

GLY

ARG

LYS

LEU

ARG

LEU

15

LYS

GLY

LYS

GLY

PHE

PRO

GLY

PRO

ALA

GLY

16

ARG

GLY

ASP

LEU

TYR

LEU

GLU

VAL

ARG

ILE

17

THR

Samples:

sample_1: C-CBD1-CBD2, [ILVMAT-sel-1H-13C_methyls; U-15N; U-2H], 1 mM; potassium chloride 75 mM; potassium phosphate 20 mM; sodium azide 0.04%

sample_2: C-CBD1-CBD2, [U-100% 13C; U-100% 15N], 1 mM; potassium chloride 75 mM; potassium phosphate 20 mM; sodium azide 0.04%

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HCH-SFNOESY	sample_1	isotropic	sample_conditions_1
3D CNH-SFNOESY	sample_1	isotropic	sample_conditions_1
3D NCH-SFNOESY	sample_1	isotropic	sample_conditions_1
3D CCH-SFNOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

Sparky, Goddard - peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

TopSpin v4.0, Bruker Biospin - collection

PSVS, Bhattacharya and Montelione - geometry optimization

NMR spectrometers:

Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks