BMRB Entry 30631

Name: Solution structure of OlvA(BC)
Published: 2019-10-14

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PDB ID: 6pqg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR30631
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of OlvA(BC)

Deposition date:

2019-07-09

Original release date:

2019-10-14

Authors:

Acedo, J.; van der Donk, W.

Citation:

Citation: Acedo, Jeella; Bothwell, Ian; An, Linna; Trouth, Abby; Frazier, Clara; van der Donk, Wilfred. "O-Methyltransferase-Mediated Incorporation of a beta-Amino Acid in Lanthipeptides" J. Am. Chem. Soc. 141, 16790-16801 (2019).
PubMed: 31568727

Assembly members:

Assembly members:
entity_1, polymer, 19 residues, 1616.841 Da.

Natural source:

Natural source: Common Name: Streptomyces olivaceus Taxonomy ID: 47716 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces olivaceus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ACGXGDGCAKXCAASCAAS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	84

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 19 residues - 1616.841 Da.

1

ALA

CYS

GLY

DBB

GLY

ASP

GLY

CYS

ALA

LYS

2

DBB

CYS

ALA

SER

CYS

ALA

SER

Samples:

sample_1: OlvA(BC) 2.5 mM; sodium phosphate 30 mM; DSS 0.001%

sample_2: OlvA(BC) 2.5 mM; sodium phosphate 30 mM; DSS 0.001%

sample_conditions_1: ionic strength: 30 mM; pH: 5.8; pressure: 760 mmHg; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 750 MHz