BMRB Entry 30608

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30608
MolProbity Validation Chart

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Title:
An order-to-disorder structural switch activates the FoxM1 transcription factor
Deposition date:
2019-05-02
Original release date:
2019-05-24
Authors:
Marceau, A.; Rubin, S.
Citation:

Citation: Marceau, A.; Brison, C.; Nerli, S.; Arsenault, H.; McShane, A.; Chen, E.; Lee, H.; Benanti, J.; Sgourakis, N.; Rubin, S.. "An order-to-disorder structural switch activates the FoxM1 transcription factor"  Elife 8, e46131-e46131 (2019).
PubMed: 31134895

Assembly members:

Assembly members:
entity_1, polymer, 94 residues, 10515.116 Da.
entity_2, polymer, 56 residues, 5950.556 Da.

Natural source:

Natural source:   Common Name: Zebrafish   Taxonomy ID: 7955   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Danio rerio

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GEFMRESPRRPIILKRRKLP FAKSTARSFPDGIRVMDHPT MPDTQVVVIPKSADLQSVIS VLTAKGKEAGPQGRNKFILL SGDTSAEEENLYFQ
entity_2: GAQAGAANRSLTEGFVLDTM NDSLSKILVDISFSGLEDED LGMGNISWSQFIPEAK

Data typeCount
13C chemical shifts189
15N chemical shifts71
1H chemical shifts71

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 94 residues - 10515.116 Da.

1   GLYGLUPHEMETARGGLUSERPROARGARG
2   PROILEILELEULYSARGARGLYSLEUPRO
3   PHEALALYSSERTHRALAARGSERPHEPRO
4   ASPGLYILEARGVALMETASPHISPROTHR
5   METPROASPTHRGLNVALVALVALILEPRO
6   LYSSERALAASPLEUGLNSERVALILESER
7   VALLEUTHRALALYSGLYLYSGLUALAGLY
8   PROGLNGLYARGASNLYSPHEILELEULEU
9   SERGLYASPTHRSERALAGLUGLUGLUASN
10   LEUTYRPHEGLN

Entity 2, entity_2 56 residues - 5950.556 Da.

1   GLYALAGLNALAGLYALAALAASNARGSER
2   LEUTHRGLUGLYPHEVALLEUASPTHRMET
3   ASNASPSERLEUSERLYSILELEUVALASP
4   ILESERPHESERGLYLEUGLUASPGLUASP
5   LEUGLYMETGLYASNILESERTRPSERGLN
6   PHEILEPROGLUALALYS

Samples:

sample_1: FoxM1 NRD, [U-13C; U-15N; U-2H], 270 uM; FoxM1 TAD, [U-13C; U-15N; U-2H], 270 uM; sodium phosphate 20 mM; potassium chloride 100 mM

sample_2: FoxM1 NRD, [U-13C; U-15N], 270 uM; FoxM1 TAD, [U-13C], 270 uM; sodium phosphate 20 mM; potassium chloride 100 mM

sample_3: FoxM1 NRD, [U-13C; U-15N; U-2H], 270 uM; FoxM1 TAD, [U-13C; U-15N; U-2H], 270 uM; sodium phosphate 20 mM; potassium chloride 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
TROSY-HNCOsample_2isotropicsample_conditions_1
TROSY-HN(CA)COsample_2isotropicsample_conditions_1
TROSY-HNCAsample_2isotropicsample_conditions_1
TROSY-HN(CO)CAsample_2isotropicsample_conditions_1
TROSY-HNCBsample_2isotropicsample_conditions_1
isotopomer-selective TOCSYsample_1isotropicsample_conditions_1
NOESY CM-NHNsample_3isotropicsample_conditions_1
NOESY HNHAro-CMHMsample_3isotropicsample_conditions_1
NOESY N-NHNsample_3isotropicsample_conditions_1
NOESY HN-NHNsample_3isotropicsample_conditions_1
methyl-HSQCsample_3isotropicsample_conditions_1
HMQCsample_3isotropicsample_conditions_1
TROSY-HN(COCA)CBsample_2isotropicsample_conditions_1
NOESY HM-CMHMsample_3isotropicsample_conditions_1
NOESY CM-CMHMsample_3isotropicsample_conditions_1
TROSY-HSQCsample_2isotropicsample_conditions_1
Dnh (NH)sample_2isotropicsample_conditions_1
DNC (NCO)sample_2isotropicsample_conditions_1

Software:

CS-ROSETTA, Shen, Vernon, Baker and Bax - refinement, structure calculation

Sparky, Goddard - chemical shift assignment, peak picking

TopSpin, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker AVANCE III HD 800 MHz
  • Varian INOVA600 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks