BMRB Entry 30592

Name: NMR structure of Orf47 from Lactococcus virus P2
Published: 2020-03-27

Click here to enlarge.

PDB ID: 6obk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30592
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR structure of Orf47 from Lactococcus virus P2

Deposition date:

2019-03-21

Original release date:

2020-03-27

Authors:

Gagne, S.

Citation:

Citation: Gagne, S.. "Structure determination of ORF47 from Lactococcus virus P2" .

Assembly members:

Assembly members:
entity_1, polymer, 43 residues, 5137.874 Da.

Natural source:

Natural source: Common Name: Lactococcus phage p2 Taxonomy ID: 254252 Superkingdom: Viruses Kingdom: not available Genus/species: Skunavirus Lactococcus phage p2

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MNKEHILAQKEVLTPIEYEH YVKHLFDIGEITKELYIELS SDL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
13C chemical shifts	114
15N chemical shifts	35
1H chemical shifts	276

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 43 residues - 5137.874 Da.

1

MET

ASN

LYS

GLU

HIS

ILE

LEU

ALA

GLN

LYS

2

GLU

VAL

LEU

THR

PRO

ILE

GLU

TYR

GLU

HIS

3

TYR

VAL

LYS

HIS

LEU

PHE

ASP

ILE

GLY

GLU

4

ILE

THR

LYS

GLU

LEU

TYR

ILE

GLU

LEU

SER

5

SER

ASP

LEU

Samples:

sample_1: entity_1 mM; potassium phosphate 20 mM; potassium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.3; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

CYANA v3.97, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks