BMRB Entry 30543

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PDB ID: 6n2m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30543
MolProbity Validation Chart

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Title:
NMR solution structure of the homodimeric, autoinhibited state of the CARD9 CARD and first coiled-coil
Deposition date:
2018-11-13
Original release date:
2019-07-12
Authors:
Holliday, M.; Fairbrother, W.; Dueber, E.
Citation:

Citation: Holliday, M.; Witt, A.; Rodriguez Gama, A.; Walters, B.; Arthur, C.; Halfmann, R.; Rohou, A.; Dueber, E.; Fairbrother, W.. "Structures of autoinhibited and polymerized forms of CARD9 reveal mechanisms of CARD9 and CARD11 activation"  Nat. Commun. 10, 3070-3070 (2019).
PubMed: 31296852

Assembly members:

Assembly members:
entity_1, polymer, 142 residues, 16116.458 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSDYENDDECWSVLEGFRVT LTSVIDPSRITPYLRQCKVL NPDDEEQVLSDPNLVIRKRK VGVLLDILQRTGHKGYVAFL ESLELYYPQLYKKVTGKEPA RVFSMIIDASGESGLTQLLM TEVMKLQKKVQDLTALLSSK DD

Data typeCount
13C chemical shifts1038
15N chemical shifts270
1H chemical shifts1149

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, 11
2entity_1, 21
3entity_2, 12
4entity_2, 22

Entities:

Entity 1, entity_1, 1 142 residues - 16116.458 Da.

1   GLYSERASPTYRGLUASNASPASPGLUCYS
2   TRPSERVALLEUGLUGLYPHEARGVALTHR
3   LEUTHRSERVALILEASPPROSERARGILE
4   THRPROTYRLEUARGGLNCYSLYSVALLEU
5   ASNPROASPASPGLUGLUGLNVALLEUSER
6   ASPPROASNLEUVALILEARGLYSARGLYS
7   VALGLYVALLEULEUASPILELEUGLNARG
8   THRGLYHISLYSGLYTYRVALALAPHELEU
9   GLUSERLEUGLULEUTYRTYRPROGLNLEU
10   TYRLYSLYSVALTHRGLYLYSGLUPROALA
11   ARGVALPHESERMETILEILEASPALASER
12   GLYGLUSERGLYLEUTHRGLNLEULEUMET
13   THRGLUVALMETLYSLEUGLNLYSLYSVAL
14   GLNASPLEUTHRALALEULEUSERSERLYS
15   ASPASP

Entity 2, entity_2, 1 - Zn - 65.409 Da.

1   ZN

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