BMRB Entry 30509

Name: HS02 - Intragenic antimicrobial peptides derived from the protein unconventional myosin 1h
Published: 2019-08-06

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PDB ID: 6mbm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30509
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

HS02 - Intragenic antimicrobial peptides derived from the protein unconventional myosin 1h

Deposition date:

2018-08-30

Original release date:

2019-08-06

Authors:

Santos, M.; Brand, G.; Oliveira, A.

Citation:

Citation: Brand, Guilherme; Ramada, Marcelo; Manickchand, Julia; Correa, Rafael; Ribeiro, Dalila; Santos, Michele; Vasconcelos, Andreanne; Abrao, Fernando; Prates, Maura; Murad, Andre; Cardozo Fh, Jose; Leite, Jose Roberto; Magalhaes, Kelly; Oliveira, Aline; Bloch, Carlos. "Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity" PLoS One 14, e0220656-e0220656 (2019).
PubMed: 31386688

Assembly members:

Assembly members:
entity_1, polymer, 16 residues, 1967.469 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KWAVRIIRKFIKGFIS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
1H chemical shifts	120

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 16 residues - 1967.469 Da.

1

LYS

TRP

ALA

VAL

ARG

ILE

ARG

LYS

PHE

2

ILE

LYS

GLY

PHE

ILE

SER

Samples:

sample_1: peptide 2 ± 0.2 uM; Dodecylphosphorylcholine-d38, D-98%, 50 ± 0.2 uM; PBS 10 ± 0.2 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

Software:

ARIA v2.3.1, Brunger, Adams, Clore, Gros, Nilges and Read, Linge, O'Donoghue and Nilges - refinement, structure calculation

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

CcpNMR v2.4, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance III HD 600 MHz