BMRB Entry 30504

Name: Solution NMR Structure of a Class I Hydrophobin from Phanerochaete carnosa
Published: 2019-08-02

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PDB ID: 6e98
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30504
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR Structure of a Class I Hydrophobin from Phanerochaete carnosa

Deposition date:

2018-07-31

Original release date:

2019-08-02

Authors:

Kenward, C.; Langelaan, D.

Citation:

Citation: Kenward, C.; Langelaan, D.. "Solution NMR Structure of a Class I Hydrophobin from Phanerochaete carnosa" .

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, 8286.277 Da.

Natural source:

Natural source: Common Name: White-rot fungus Taxonomy ID: 650164 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Phanerochaete carnosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli DH1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSWGQCSTGSIQCCQNVVPG DSDLGTLLLDELGIVLEDPT VLIGDGCDPITVAGSSDACS ATAVCCSDNNVSGVIAIGCL PVTL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	326
15N chemical shifts	85
1H chemical shifts	519

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 84 residues - 8286.277 Da.

1

GLY

SER

TRP

GLY

GLN

CYS

SER

THR

GLY

SER

2

ILE

GLN

CYS

GLN

ASN

VAL

PRO

GLY

3

ASP

SER

ASP

LEU

GLY

THR

LEU

ASP

4

GLU

LEU

GLY

ILE

VAL

LEU

GLU

ASP

PRO

THR

5

VAL

LEU

ILE

GLY

ASP

GLY

CYS

ASP

PRO

ILE

6

THR

VAL

ALA

GLY

SER

ASP

ALA

CYS

SER

7

ALA

THR

ALA

VAL

CYS

SER

ASP

ASN

8

VAL

SER

GLY

VAL

ILE

ALA

ILE

GLY

CYS

LEU

9

PRO

VAL

THR

LEU

Samples:

sample_1: PC1, [U-13C; U-15N], 800 uM; MES 20 mM; sodium chloride 50 mM; sodium azide 1 mM

sample_2: PC1, [U-15N], 110 uM; MES 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 303.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

ARIA v2.3.1, Linge, O'Donoghue and Nilges - refinement, structure calculation

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

Bruker AvanceIII 800 MHz
Bruker AvanceIII 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks