BMRB Entry 30475

Name: Solution structure of ZmD32
Published: 2019-05-07

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PDB ID: 6dmz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30475
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of ZmD32

Deposition date:

2018-06-05

Original release date:

2019-05-07

Authors:

Harvey, P.; Craik, D.

Citation:

Citation: Kerenga, Bomai; McKenna, James; Harvey, Peta; Quimbar, Pedro; Garcia-Ceron, Donovan; Lay, Fung; Phan, Thanh Kha; Veneer, Prem; Vasa, Shaily; Parisi, Kathy; Shafee, Thomas; van der Weerden, Nicole; Hulett, Mark; Craik, David; Anderson, Marilyn; Bleackley, Mark. "Salt-Tolerant Antifungal and Antibacterial Activities of the Corn Defensin ZmD32." Front. Microbiol. 10, 795-795 (2019).
PubMed: 31031739

Assembly members:

Assembly members:
entity_1, polymer, 47 residues, 5479.359 Da.

Natural source:

Natural source: Common Name: Maize Taxonomy ID: 4577 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Zea mays

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Komagataella pastoris

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RTCQSQSHRFRGPCLRRSNC ANVCRTEGFPGGRCRGFRRR CFCTTHC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	99
15N chemical shifts	37
1H chemical shifts	279

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 47 residues - 5479.359 Da.

1

ARG

THR

CYS

GLN

SER

GLN

SER

HIS

ARG

PHE

2

ARG

GLY

PRO

CYS

LEU

ARG

SER

ASN

CYS

3

ALA

ASN

VAL

CYS

ARG

THR

GLU

GLY

PHE

PRO

4

GLY

ARG

CYS

ARG

GLY

PHE

ARG

5

CYS

PHE

CYS

THR

HIS

CYS

Samples:

sample_1: peptide 1 mM

sample_2: peptide 1 mM

sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H ECOSY	sample_2	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CcpNMR, CCPN - chemical shift assignment

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

Bruker AvanceIII 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks