BMRB Entry 30449

Name: HRFLRH peptide NMR structure in the presence of Zn(II)
Published: 2018-06-26

Click here to enlarge.

PDB ID: 6cxr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30449
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

HRFLRH peptide NMR structure in the presence of Zn(II)

Deposition date:

2018-04-03

Original release date:

2018-06-26

Authors:

Pires, D.; Arake, L.; Silva, L.; Lopez-Castillo, A.; Prates, M.; Nascimento, C.; Bloch Jr, C.

Citation:

Citation: Pires, D.; Arake, L.; Silva, L.; Lopez-Castillo, A.; Prates, M.; Nascimento, C.; Bloch Jr, C.. "A Previously Undescribed Hexapeptide His-Arg-Phe-Leu-Arg-His-NH2 From Amphibian Skin Secretion shows CO2 and Metal Biding Affinities" Peptides 106, 37-44 (2018).
PubMed: 29933027

Assembly members:

Assembly members:
entity_1, polymer, 7 residues, 867.036 Da.

Natural source:

Natural source: Common Name: Mato Grosso leaf frog Taxonomy ID: 536631 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Phyllomedusa centralis

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: HRFLRHX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	28
1H chemical shifts	48

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 7 residues - 867.036 Da.

1

HIS

ARG

PHE

LEU

ARG

HIS

NH2

Samples:

sample_1: HIS-ARG-PHE-LEU-ARG-HIS, [U-13C], 2 mM; Zn(II) 2 mM

sample_conditions_1: ionic strength: 2 mM; pH: 4.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker AvanceIII 800 MHz
Bruker AvanceIII 800 MHz