BMRB Entry 30417

Title:
NMR Structure of Salmonella Type III Secretion system protein OrgC   PubMed: 30015613
Deposition date:
2018-02-26
Original release date:
2018-07-24
Authors:
Dey, S.; De Guzman, R.
Citation:

Citation: Kato, Junya; Dey, Supratim; Soto, Jose; Butan, Carmen; Wilkinson, Mason; De Guzman, Roberto; Galan, Jorge. "A protein secreted by the Salmonella type III secretion system controls needle filament assembly"  Elife 7, e35886-e35886 (2018).

Assembly members:

Assembly members:
entity_1, polymer, 131 residues, 14293.888 Da.

Natural source:

Natural source:   Common Name: Salmonella enterica   Taxonomy ID: 59201   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Salmonella enterica

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Experimental source:

Natural source:   Common Name: Salmonella enterica   Taxonomy ID: 59201   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Salmonella enterica

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Data sets:
Data typeCount
13C chemical shifts238
15N chemical shifts123
1H chemical shifts245

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 131 residues - 14293.888 Da.

1   GLYHISMETVALSERLEUSERALAARGALA
2   ALAMETLEUASNASNMETASPSERALAPRO
3   LEUSERASNGLYGLYASPVALASPLEUTYR
4   ASPALAPHETYRGLNARGLEULEUALALEU
5   PROGLUSERALASERSERGLUTHRLEULYS
6   ASPSERILETYRGLNGLUMETASNALAPHE
7   LYSASPPROASNSERGLYASPSERALAPHE
8   VALSERPHEGLUGLNGLNTHRALAMETLEU
9   GLNASNMETLEUALALYSVALGLUPROGLY
10   THRHISLEUTYRGLUALALEUASNGLYVAL
11   LEUVALGLYSERMETASNALAGLNSERGLN
12   METTHRSERTRPMETGLNGLUILEILELEU
13   SERGLYGLYGLUASNLYSGLUALAILEASP
14   TRP

Samples:

sample_1: OrgC, [U-99% 15N], 1.2 mM; OrgC, [U-13C; U-15N], 1.2 mM; OrgC, [U-13C], 1.2 mM; MES 2 mM; NaCl 75 mM

sample_conditions_1: ionic strength: 75 mM; pH: 6.5 pH*; pressure: 1 mbar; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HMQC NOESYsample_1isotropicsample_conditions_1
HSQC NOESYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks