BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30404

Title: NMR structure of the Rous sarcoma virus matrix protein (M-domain) in the presence of myo-inositol hexakisphosphate

Deposition date: 2018-02-11 Original release date: 2018-10-15

Authors: Vlach, J.; Saad, J.

Citation: Vlach, J.; Eastep, G.; Ghanam, R.; Saad, J.. "Interactions of ASV matrix protein with phospholipids"  .

Assembly members:
entity_1, polymer, 87 residues, 9209.771 Da.

Natural source:   Common Name: RSV-PrC   Taxonomy ID: 11888   Superkingdom: Viruses   Kingdom: not available   Genus/species: Alpharetrovirus Rous sarcoma virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: SEAVIKVISSACKTYCGKTS PSKKEIGAMLSLLQKEGLLM SPSDLYSPGSWDPITAALSQ RAMILGKSGELKTWGLVLGA LKAAREE

Data sets:
Data typeCount
13C chemical shifts379
15N chemical shifts87
1H chemical shifts615

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 87 residues - 9209.771 Da.

1   SERGLUALAVALILELYSVALILESERSER
2   ALACYSLYSTHRTYRCYSGLYLYSTHRSER
3   PROSERLYSLYSGLUILEGLYALAMETLEU
4   SERLEULEUGLNLYSGLUGLYLEULEUMET
5   SERPROSERASPLEUTYRSERPROGLYSER
6   TRPASPPROILETHRALAALALEUSERGLN
7   ARGALAMETILELEUGLYLYSSERGLYGLU
8   LEULYSTHRTRPGLYLEUVALLEUGLYALA
9   LEULYSALAALAARGGLUGLU

Samples:

sample_1: Matrix protein, [U-95% 15N], 0.5 mM; sodium phosphate 50 mM; tcep 2 mM; IP6 2 mM

sample_2: Matrix protein, [U-95% 13C; U-95% 15N], 0.5 mM; sodium phosphate 50 mM; tcep 2 mM; IP6 2 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

unio, Herrmann - structure calculation

NMR spectrometers:

  • Bruker AvanceII 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts