BMRB Entry 30379

NMR structure of Sodium/Calcium Exchanger 1 (NCX1) Two-helix Bundle (THB) domain
Deposition date:
Original release date:
Yuan, J.; Yuan, C.; Bruschweiler, R.

Citation: Yuan, J.; Yuan, C.; Xie, M.; Yu, L.; Bruschweiler-Li, L.; Bruschweiler, R.. "The Intracellular Loop of the Na+/Ca2+ Exchanger Contains a Novel Two-Helix Bundle Domain"  Biochemistry 57, 5096-5104 (2018).
PubMed: 29898361

Assembly members:

Assembly members:
entity_1, polymer, 57 residues, 6788.544 Da.

Natural source:

Natural source:   Common Name: Dog   Taxonomy ID: 9615   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Canis lupus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli K-12   Vector: pTBSG1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts258
15N chemical shifts62
1H chemical shifts380

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all


Entity Assembly IDEntity NameEntity ID


Entity 1, entity_1 57 residues - 6788.544 Da.



sample_1: NCX1_THB, [U-15N], 300 uM; sodium chloride 100 mM; sodium phosphate 20 mM

sample_2: sodium chloride 100 mM; sodium phosphate 20 mM; NCX1 THB, [U-13C; U-15N], 300 uM

sample_3: sodium chloride 100 mM; sodium phosphate 20 mM; NCX1 THB, [U-13C; U-15N], 300 uM

sample_4: sodium chloride 100 mM; sodium phosphate 20 mM; NCX1 THB 300 uM

sample_5: sodium chloride 100 mM; sodium phosphate 20 mM; NCX1 THB, [U-10% 13C], 300 uM

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K


NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCACOsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D H(CO)NH-TOCSYsample_2isotropicsample_conditions_1
3D (H)CC(CO)NH-TOCSYsample_2isotropicsample_conditions_1
2D aromatic TROSY-HSQCsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_4isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
2D DQF-COSYsample_4isotropicsample_conditions_1
2D 10%-13C CT-HSQCsample_5isotropicsample_conditions_1
3D 15N-edited NOESYsample_2isotropicsample_conditions_1
3D 15N-edited CNH-NOESYsample_2isotropicsample_conditions_1
3D 13C-edited NOESYsample_3isotropicsample_conditions_1
3D 13C-edited CCH-NOESYsample_3isotropicsample_conditions_1


NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - data analysis

NMRView, Johnson, One Moon Scientific - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

NMR spectrometers:

  • Bruker AvanceIII 850 MHz
  • Bruker AvanceIII 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks