BMRB Entry 30371

Name: NMR Structural and biophysical functional analysis of intracellular loop 5 of the NHE1 isoform of the Na+/H+ exchanger.
Published: 2018-11-06

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PDB ID: 6bjf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30371
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Structural and biophysical functional analysis of intracellular loop 5 of the NHE1 isoform of the Na+/H+ exchanger.

Deposition date:

2017-11-06

Original release date:

2018-11-06

Authors:

McKay, R.; Wong, K.; Towle, K.; Fliegel, L.

Citation:

Citation: Wong, Ka Yee; McKay, Ryan; Liu, Yongsheng; Towle, Kaitlyn; Elloumi, Yesmine; Li, Xiuju; Quan, Sicheng; Dutta, Debajyoti; Sykes, Brian; Fliegel, Larry. "Diverse residues of intracellular loop 5 of the Na+/H+ exchanger modulate proton sensing, expression, activity and targeting" Biochim. Biophys. Acta Biomembr. 1861, 191-200 (2019).
PubMed: 30071192

Assembly members:

Assembly members:
entity_1, polymer, 13 residues, 1624.988 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GLTWFINKFRIVK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
1H chemical shifts	97

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 13 residues - 1624.988 Da.

1

GLY

LEU

THR

TRP

PHE

ILE

ASN

LYS

PHE

ARG

2

ILE

VAL

LYS

Samples:

sample_1: peptide 5 ± 0.5 mM; Na+ 440 mM

sample_conditions_1: ionic strength: 440 mM; pH: 6; pressure: 960 mbar; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D Proton	sample_1	isotropic	sample_conditions_1
2D NOESY	sample_1	isotropic	sample_conditions_1
2D HSQC	sample_1	isotropic	sample_conditions_1
2D TOCSY	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ v4.2, Agilent - collection

NMRPipe v8.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - peak picking

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation

NMR spectrometers:

Varian VNMRS 600 MHz