BMRB Entry 30311

Name: Solution structure of phage displayed derived peptide inhibitor of frizzled 7 receptor
Published: 2018-04-11

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PDB ID: 5w96
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30311
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of phage displayed derived peptide inhibitor of frizzled 7 receptor

Deposition date:

2017-06-22

Original release date:

2018-04-11

Authors:

Fu, Y.

Citation:

Citation: Nile, Aaron; de Sousa E Melo, Felipe; Mukund, Susmith; Piskol, Robert; Hansen, Simon; Zhou, Lijuan; Zhang, Yingnan; Fu, Yue; Gogol, Emily; Komuves, Laszlo; Modrusan, Zora; Angers, Stephane; Franke, Yvonne; Koth, Christopher; Fairbrother, Wayne; Wang, Weiru; de Sauvage, Frederic; Hannoush, Rami. "A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells" Nat. Chem. Biol. 14, 582-590 (2018).
PubMed: 29632413

Assembly members:

Assembly members:
Fz7 binding peptide, polymer, 14 residues, 1755.986 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 32644 Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fz7 binding peptide: LPSDDLEFWCHVMY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	178

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1, chain A	1
2	entity_1, chain B	1

Entities:

Entity 1, entity_1, chain A 14 residues - 1755.986 Da.

1

LEU

PRO

SER

ASP

LEU

GLU

PHE

TRP

CYS

2

HIS

VAL

MET

TYR

Samples:

sample_1: Fz binding peptide 1 mM; acentonitrile 10%; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

Software:

Analysis, CCPN - chemical shift assignment, data analysis, peak picking

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMR spectrometers:

Bruker ultrashield plus 600 MHz