BMRB Entry 30306

Name: Cytokine-like Stress Response Peptide-2 in Manduca Sexta
Published: 2017-07-07

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PDB ID: 5w54
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30306
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Cytokine-like Stress Response Peptide-2 in Manduca Sexta

Deposition date:

2017-06-14

Original release date:

2017-07-07

Authors:

Schrag, L.; Herrera, A.; Prakash, O.

Citation:

Citation: Schrag, L.; Cao, X.; Herrera, A.; Wang, Y.; Jiang, H.; Prakash, O.. "Solution Structure and Expression Profile of an Insect Cytokine: Manduca sexta Stress Response Peptide-2." Protein Pept. Lett. 24, 3-11 (2017).
PubMed: 27903232

Assembly members:

Assembly members:
entity_1, polymer, 25 residues, 2758.163 Da.

Natural source:

Natural source: Common Name: tobacco hornworm Taxonomy ID: 7130 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Manduca sexta

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: FGVKDGKCPSGRVRRLGICV PDDDY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	148

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 25 residues - 2758.163 Da.

1

PHE

GLY

VAL

LYS

ASP

GLY

LYS

CYS

PRO

SER

2

GLY

ARG

VAL

ARG

LEU

GLY

ILE

CYS

VAL

3

PRO

ASP

TYR

Samples:

sample_1: Stress Response Peptide-2 2.0 mM

sample_conditions_1: pH: 2.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D DQF-COSY	sample_1	anisotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	anisotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	anisotropic	sample_conditions_1

Software:

CNS v1.3, Brunger A. T. et.al. - refinement, structure calculation

CcpNMR, CCPN - chemical shift assignment, peak picking

Procheck v3.4, Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Thornton - refinement

VNMRJ v3.2, Varian - collection

NMR spectrometers:

Varian VNMRS 500 MHz