BMRB Entry 30071

Name: Calcium-loaded EF-hand domain of L-plastin
Published: 2017-04-13

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PDB ID: 5joj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30071
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Calcium-loaded EF-hand domain of L-plastin

Deposition date:

2016-05-02

Original release date:

2017-04-13

Authors:

Ishida, Hiroaki; Jensen, K.; Woodman, G.; Hyndman, Eric; Vogel, Hans

Citation:

Citation: Ishida, Hiroaki; Jensen, Katharine; Woodman, Andrew; Hyndman, M. Eric; Vogel, Hans. "The Calcium-Dependent Switch Helix of L-Plastin Regulates Actin Bundling." Sci. Rep. 7, 40662-40662 (2017).
PubMed: 28145401

Assembly members:

Assembly members:
Plastin-2, polymer, 97 residues, 10968.452 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Plastin-2: MARGSVSDEEMMELREAFAK VDTDGNGYISFNELNDLFKA ACLPLPGYRVREITENLMAT GDLDQDGRISFDEFIKIFHG LKSTDVAKTFRKAINKK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	284
15N chemical shifts	95
1H chemical shifts	94

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	CALCIUM ION	2
3	CALCIUM ION	2

Entities:

Entity 1, entity_1 97 residues - 10968.452 Da.

1

MET

ALA

ARG

GLY

SER

VAL

SER

ASP

GLU

2

MET

GLU

LEU

ARG

GLU

ALA

PHE

ALA

LYS

3

VAL

ASP

THR

ASP

GLY

ASN

GLY

TYR

ILE

SER

4

PHE

ASN

GLU

LEU

ASN

ASP

LEU

PHE

LYS

ALA

5

ALA

CYS

LEU

PRO

LEU

PRO

GLY

TYR

ARG

VAL

6

ARG

GLU

ILE

THR

GLU

ASN

LEU

MET

ALA

THR

7

GLY

ASP

LEU

ASP

GLN

ASP

GLY

ARG

ILE

SER

8

PHE

ASP

GLU

PHE

ILE

LYS

ILE

PHE

HIS

GLY

9

LEU

LYS

SER

THR

ASP

VAL

ALA

LYS

THR

PHE

10

ARG

LYS

ALA

ILE

ASN

LYS

Entity 2, CALCIUM ION - 40.078 Da.

1

CA

Samples:

sample_1: EF-hand domain of L-plastin, [U-98% 15N], 1.0 mM; H2O 90%; D2O 10%

sample_2: EF-hand domain of L-plastin, [U-99% 13C; U-98% 15N], 0.5 mM; H2O 90%; D2O 10%

sample_3: EF-hand domain of L-plastin, [U-99% 13C; U-98% 15N], 0.5 mM; D2O 100%

sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_3	isotropic	sample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks