BMRB Entry 30032

Name: Solution Structure of Miz-1 Zinc Finger 13
Published: 2016-03-28

Click here to enlarge.

PDB ID: 5ion
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30032
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution Structure of Miz-1 Zinc Finger 13

Deposition date:

2016-03-08

Original release date:

2016-03-28

Authors:

Tremblay, C.; Bedard, M.; Lavigne, P.

Citation:

Citation: Tremblay, C.; Bedard, M.; Bonin, MA.; Lavigne, P.. "Solution structure of the 13th C2H2 Zinc Finger of Miz-1" Biochem. Biophys. Res. Commun. 473, 471-475 (2016).
PubMed: 26972249

Assembly members:

Assembly members:
entity_1, polymer, 29 residues, 3232.629 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: HILYACDSCGDKFLDANSLA QHVRIHTAQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	65
15N chemical shifts	22
1H chemical shifts	178

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Entities:

Entity 1, entity_1 29 residues - 3232.629 Da.

1

HIS

ILE

LEU

TYR

ALA

CYS

ASP

SER

CYS

GLY

2

ASP

LYS

PHE

LEU

ASP

ALA

ASN

SER

LEU

ALA

3

GLN

HIS

VAL

ARG

ILE

HIS

THR

ALA

GLN

Entity 2, entity_2 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: ZF 13 0.75 mM; ZnCl2 2 mM; KCl 50 mM; Bis Tris, [U-2H], 10 mM; TECP 2 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

ARIA v2.2, Linge, O'Donoghue and Nilges - structure calculation

CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CcpNMR_Analysis, CCPN - chemical shift assignment

DANGLE v1.1, CCPN - data analysis

NMRPipe v7.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian Unity Inova 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks