BMRB Entry 30031

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30031
MolProbity Validation Chart

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Title:
Solution Structure of the Microtubule-Targeting COS Domain of MID1
Deposition date:
2016-03-05
Original release date:
2016-07-06
Authors:
Wright, K.; Du, H.; Dagnachew, M.; Massiah, M.
Citation:

Citation: Wright, K.; Du, H.; Dagnachew, M.; Massiha, M.. "Solution structure of the microtubule-targeting COS domain of MID1"  FEBS J. 283, 3089-3102 (2016).
PubMed: 27367845

Assembly members:

Assembly members:
entity_1, polymer, 60 residues, 6926.834 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: LKENDHARFLQTAKNITERV SMATASSQVLIPEINLNDTF DTFALDFSREKKLLECLDYL

Data sets:
Data typeCount
13C chemical shifts231
15N chemical shifts52
1H chemical shifts379

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 60 residues - 6926.834 Da.

1   LEULYSGLUASNASPHISALAARGPHELEU
2   GLNTHRALALYSASNILETHRGLUARGVAL
3   SERMETALATHRALASERSERGLNVALLEU
4   ILEPROGLUILEASNLEUASNASPTHRPHE
5   ASPTHRPHEALALEUASPPHESERARGGLU
6   LYSLYSLEULEUGLUCYSLEUASPTYRLEU

Samples:

sample_1: entity_1 mM; TRIS 50 mM; beta-mercaptoethanol 10 mM; sodium azide 0.2%

sample_conditions_1: ionic strength: 50 mM; pH: 7.8; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Agilent Agilent DD2 600 MHz 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks