BMRB Entry 28136

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28136

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Title:
Resonance Assignments for the Rabies Phosphoprotein RavP
Deposition date:
2020-07-03
Original release date:
2020-07-29
Authors:
Jespersen, Nathan; Leyrat, Cedric; Gerard, Francine; Bourhis, Jean-Marie; Blondel, Danielle; Jamin, Marc; Barbar, Elisar
Citation:

Citation: Jespersen, Nathan; Leyrat, Cedric; Gerard, Francine; Bourhis, Jean-Marie; Blondel, Danielle; Jamin, Marc; Barbar, Elisar. "The LC8-RavP Ensemble Structure Evinces A Role for LC8 in Regulating Lyssavirus Polymerase Functionality"  J. Mol. Biol. 431, 4959-4977 (2019).
PubMed: 31634467

Assembly members:

Assembly members:
Rabies_Phosphoprotein_(RavP), polymer, 301 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rabies lyssavirus   Taxonomy ID: 11292   Superkingdom: Viruses   Kingdom: not available   Genus/species: Rabies Virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24d+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rabies_Phosphoprotein_(RavP): GAHMMSKIFVNPSAIRAGLA DLEMAEETVDLINRNIEDNQ AHLQGEPIEVDNLPEDMGRL HLDGGKSSNPGEMAKVGEGK YREDFQMDEGEDPSLLFQSY LDNVGVQIVRQIRSGERFLK IWSQTVEEIISYVAVNFPNP PGKSSEDKSTQTTGRELKKE TTPTPSQRESQSSKARMAAQ TASGPPALEWSATNEEDDLS VEAEIAHQIAESFSKKYKFP SRSSGILLYNFEQLKMNLDD IVKEAKNVPGVTRLARDGSK LPLRCVLGWVALANSKKFQL LVESNKLSKIMQDDLNRYTS C

Data sets:
Data typeCount
13C chemical shifts102
15N chemical shifts198
1H chemical shifts198

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RavP, chain A1
2RavP, chain B1

Entities:

Entity 1, RavP, chain A 301 residues - Formula weight is not available

GAHM at N-terminus is from cut tag.

1   GLYALAHISMETMETSERLYSILEPHEVAL
2   ASNPROSERALAILEARGALAGLYLEUALA
3   ASPLEUGLUMETALAGLUGLUTHRVALASP
4   LEUILEASNARGASNILEGLUASPASNGLN
5   ALAHISLEUGLNGLYGLUPROILEGLUVAL
6   ASPASNLEUPROGLUASPMETGLYARGLEU
7   HISLEUASPGLYGLYLYSSERSERASNPRO
8   GLYGLUMETALALYSVALGLYGLUGLYLYS
9   TYRARGGLUASPPHEGLNMETASPGLUGLY
10   GLUASPPROSERLEULEUPHEGLNSERTYR
11   LEUASPASNVALGLYVALGLNILEVALARG
12   GLNILEARGSERGLYGLUARGPHELEULYS
13   ILETRPSERGLNTHRVALGLUGLUILEILE
14   SERTYRVALALAVALASNPHEPROASNPRO
15   PROGLYLYSSERSERGLUASPLYSSERTHR
16   GLNTHRTHRGLYARGGLULEULYSLYSGLU
17   THRTHRPROTHRPROSERGLNARGGLUSER
18   GLNSERSERLYSALAARGMETALAALAGLN
19   THRALASERGLYPROPROALALEUGLUTRP
20   SERALATHRASNGLUGLUASPASPLEUSER
21   VALGLUALAGLUILEALAHISGLNILEALA
22   GLUSERPHESERLYSLYSTYRLYSPHEPRO
23   SERARGSERSERGLYILELEULEUTYRASN
24   PHEGLUGLNLEULYSMETASNLEUASPASP
25   ILEVALLYSGLUALALYSASNVALPROGLY
26   VALTHRARGLEUALAARGASPGLYSERLYS
27   LEUPROLEUARGCYSVALLEUGLYTRPVAL
28   ALALEUALAASNSERLYSLYSPHEGLNLEU
29   LEUVALGLUSERASNLYSLEUSERLYSILE
30   METGLNASPASPLEUASNARGTYRTHRSER
31   CYS

Samples:

sample_1: Rabies Phosphoprotein (RavP), [U-99% 13C; U-99% 15N], 150 uM; DSS, [U-2H], 100 uM; sodium azide 1 mM; sodium chloride 100 mM; MES 25 mM; EDTA 5 mM; beta-mercaptoethanol 5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks