BMRB Entry 28133

Name: Backbone 1H, 13C, 15N, and CB chemical shift assignments for the PDE6 inhibitory gamma-subunit (1-58)
Published: 2020-09-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28133

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, 15N, and CB chemical shift assignments for the PDE6 inhibitory gamma-subunit (1-58)

Deposition date:

2020-06-15

Original release date:

2020-09-16

Authors:

Gupta, Richa; Liu, Yong; Wang, Huanchen; Nordyke, Christopher; Puterbaugh, Ryan; Varga, Krisztina; Chu, Feixia; Ke, Hengming; Vashisth, Harish; Cote, Rick

Citation:

Citation: Gupta, Richa; Liu, Yong; Wang, Huanchen; Nordyke, Christopher; Puterbaugh, Ryan; Cui, Wenjun; Varga, Krisztina; Chu, Feixia; Ke, Hengming; Vashisth, Harish; Cote, Rick. "Structural Analysis of the Regulatory GAF Domains of cGMP Phosphodiesterase Elucidates the Allosteric Communication Pathway" J. Mol. Biol. 432, 5765-5783 (2020).
PubMed: 32898583

Assembly members:

Assembly members:
PDE6_inhibitory_gamma-subunit, polymer, 58 residues, 6255.13 Da.

Natural source:

Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDE6_inhibitory_gamma-subunit: MSENPTTNLTTGDAPTGPTT PRKGPPKFKQRQTRQFKSKP PKKGVKGFGDDIPGMEGL

Data sets:

assigned_chemical_shifts
- Pgamma_PDE6_NMRSTAR

Data type	Count
13C chemical shifts	146
15N chemical shifts	47
1H chemical shifts	47

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDE6 inhibitory gamma-subunit (1-58)	1

Entities:

Entity 1, PDE6 inhibitory gamma-subunit (1-58) 58 residues - 6255.13 Da.

1

MET

SER

GLU

ASN

PRO

THR

ASN

LEU

THR

2

THR

GLY

ASP

ALA

PRO

THR

GLY

PRO

THR

3

PRO

ARG

LYS

GLY

PRO

LYS

PHE

LYS

GLN

4

ARG

GLN

THR

ARG

GLN

PHE

LYS

SER

LYS

PRO

5

PRO

LYS

GLY

VAL

LYS

GLY

PHE

GLY

ASP

6

ASP

ILE

PRO

GLY

MET

GLU

GLY

LEU

Samples:

sample_1: PDE6 inhibitory gamma-subunit (1-58), [U-98% 13C; U-98% 15N], 50 uM; TRIS 20 mM; sodium chloride 50 mM; magnesium chloride 1 mM; D2O, [U-2H], 5 % v/v; sodium azide 1 mM; DSS 200 uM

sample_1_conditions: pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_1_conditions
3D HNCA	sample_1	isotropic	sample_1_conditions
3D HNCACB	sample_1	isotropic	sample_1_conditions
3D HNCO	sample_1	isotropic	sample_1_conditions

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance III HD 700 MHz
Bruker Avance NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks