BMRB Entry 28131

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28131

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Title:
1H 15N 13C Resonance Assignments of Receptor Binding Domain 2 of CDTb (757-876)
Deposition date:
2020-06-10
Original release date:
2021-07-16
Authors:
Cook, Mary; Varney, Kristen; Godoy-Ruiz, Raquel; Weber, David
Citation:

Citation: Cook, Mary; Varney, Kristen; Godoy-Ruiz, Raquel; Weber, David. "1HN, 13C, AND 15N RESONANCE ASSIGNMENTS OF RECEPTOR BINDING DOMAIN 2 (CDTB, RESIDUES 757-876)"  Biomol. NMR Assign. 15, 35-39 (2021).
PubMed: 33034833

Assembly members:

Assembly members:
CDTb_receptor_binding_domain_2, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Clostridium difficile   Taxonomy ID: 1496   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium difficile

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CDTb_receptor_binding_domain_2: DDDDKTDQEIMDAHKIYFAD LNFNPSTGNTYINGMYFAPT QTNKEALDYIQKYRVEATLQ YSGFKDIGTKDKEMRNYLGD PNQPKTNYVNLRSYFTGGEN IMTYKKLRIYAITPDDRELL VLSVD

Data sets:
Data typeCount
13C chemical shifts431
15N chemical shifts116
1H chemical shifts574

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1RBD21

Entities:

Entity 1, RBD2 125 residues - Formula weight is not available

6 THR corresponds to 757 THR in full-length CDTb; start author sequence numbering at 6 THR. (There is a HIS-tag present).

1   ASPASPASPASPLYSTHRASPGLNGLUILE
2   METASPALAHISLYSILETYRPHEALAASP
3   LEUASNPHEASNPROSERTHRGLYASNTHR
4   TYRILEASNGLYMETTYRPHEALAPROTHR
5   GLNTHRASNLYSGLUALALEUASPTYRILE
6   GLNLYSTYRARGVALGLUALATHRLEUGLN
7   TYRSERGLYPHELYSASPILEGLYTHRLYS
8   ASPLYSGLUMETARGASNTYRLEUGLYASP
9   PROASNGLNPROLYSTHRASNTYRVALASN
10   LEUARGSERTYRPHETHRGLYGLYGLUASN
11   ILEMETTHRTYRLYSLYSLEUARGILETYR
12   ALAILETHRPROASPASPARGGLULEULEU
13   VALLEUSERVALASP

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