BMRB Entry 28109

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28109

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for human P[4] rotavirus strain BM5265
Deposition date:
2020-03-26
Original release date:
2021-08-06
Authors:
Xu, Shenyuan; Jiang, Xi; Kennedy, Michael
Citation:

Citation: Xu, Shenyuan; McGinnis, Kristen Rose; Liu, Yang; Huang, Pengwei; Tan, Ming; Stuckert, Michael Robert; Burnside, Riley Erin; Jacob, Elsa Grace; Ni, Shuisong; Jiang, Xi; Kennedy, Michael. "Structural basis of P[II] rotavirus evolution and host ranges under selection of histo-blood group antigens"  Proc. Natl. Acad. Sci. U. S. A. 118, e2107963118-e2107963118 (2021).
PubMed: 34475219

Assembly members:

Assembly members:
entity_1, polymer, 160 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: VLDGPYQPTTFKPPNDYWLL ISSNTDGVVYESTNNSDFWT AVIAVEPHVSQTNRQYVLFG ENKQFNVENNSDKWKFFEMF KGSGQSDFSNRRTLTSNNRL VGMLKYGGRVWTFHGETPRA TTDSSNTADLNNISIIIHSE FYIIPRSQESKCNEYINNGL

Data sets:
Data typeCount
13C chemical shifts453
15N chemical shifts141
1H chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P[4] VP8*1

Entities:

Entity 1, P[4] VP8* 160 residues - Formula weight is not available

1   VALLEUASPGLYPROTYRGLNPROTHRTHR
2   PHELYSPROPROASNASPTYRTRPLEULEU
3   ILESERSERASNTHRASPGLYVALVALTYR
4   GLUSERTHRASNASNSERASPPHETRPTHR
5   ALAVALILEALAVALGLUPROHISVALSER
6   GLNTHRASNARGGLNTYRVALLEUPHEGLY
7   GLUASNLYSGLNPHEASNVALGLUASNASN
8   SERASPLYSTRPLYSPHEPHEGLUMETPHE
9   LYSGLYSERGLYGLNSERASPPHESERASN
10   ARGARGTHRLEUTHRSERASNASNARGLEU
11   VALGLYMETLEULYSTYRGLYGLYARGVAL
12   TRPTHRPHEHISGLYGLUTHRPROARGALA
13   THRTHRASPSERSERASNTHRALAASPLEU
14   ASNASNILESERILEILEILEHISSERGLU
15   PHETYRILEILEPROARGSERGLNGLUSER
16   LYSCYSASNGLUTYRILEASNASNGLYLEU

Samples:

sample_1: P[4] VP8*, [U-13C; U-99% 15N], 0.6 mM; sodium chloride 150 mM; potassium chloride 2.7 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM

sample_conditions_1: ionic strength: 200 mM; pH: 7.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks