BMRB Entry 28091

Name: Retinoblastoma-like protein 1 / p107
Published: 2021-09-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28091

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Retinoblastoma-like protein 1 / p107

Deposition date:

2020-03-03

Original release date:

2021-09-20

Authors:

Wang, Xinru; Page, Rebecca; Peti, Wolfgang

Citation:

Citation: Fowle, Holly; Zhao, Ziran; Xu, Qifang; Wasserman, Jason; Wang, Xinru; Adeyemi, Mary; Feiser, Felicity; Kurimchak, Alison; Atar, Diba; McEwan, Brennan; Kettenbach, Arminja; Page, Rebecca; Peti, Wolfgang; Dunbrack, Roland; Grana, Xavier. "B55/PP2A substrate recruitment as defined by the retinoblastoma-related protein p107" eLife 10, e63181-e63181 (2021).
PubMed: 34661528

Assembly members:

Assembly members:
p107, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTHMT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p107: GHMPMSPLMHPRVKEVRTDS GSLRRDMQPLSPISVHERYS SPTAGSAKRRLFGEDPPKEM LMDKIITEGTKLKIAPSS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	147
15N chemical shifts	65
1H chemical shifts	65

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p107	1

Entities:

Entity 1, p107 78 residues - Formula weight is not available

Residue 1-2 represent a cloning artifact. Residue 3-78 represent human p107 residue 612-687.

1

GLY

HIS

MET

PRO

MET

SER

PRO

LEU

MET

HIS

2

PRO

ARG

VAL

LYS

GLU

VAL

ARG

THR

ASP

SER

3

GLY

SER

LEU

ARG

ASP

MET

GLN

PRO

LEU

4

SER

PRO

ILE

SER

VAL

HIS

GLU

ARG

TYR

SER

5

SER

PRO

THR

ALA

GLY

SER

ALA

LYS

ARG

6

LEU

PHE

GLY

GLU

ASP

PRO

LYS

GLU

MET

7

LEU

MET

ASP

LYS

ILE

THR

GLU

GLY

THR

8

LYS

LEU

LYS

ILE

ALA

PRO

SER

Samples:

sample_1: p107, [U-99% 13C; U-99% 15N], 0.7 mM; sodium chloride 50 mM; TCEP 0.5 mM; sodium phosphate 20 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 70 mM; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

cara v1.9.2, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Neo 600 MHz