BMRB Entry 28091

Title:
Retinoblastoma-like protein 1 / p107
Deposition date:
2020-03-03
Original release date:
2021-09-20
Authors:
Wang, Xinru; Page, Rebecca; Peti, Wolfgang
Citation:

Citation: Fowle, Holly; Zhao, Ziran; Xu, Qifang; Wasserman, Jason; Wang, Xinru; Adeyemi, Mary; Feiser, Felicity; Kurimchak, Alison; Atar, Diba; McEwan, Brennan; Kettenbach, Arminja; Page, Rebecca; Peti, Wolfgang; Dunbrack, Roland; Grana, Xavier. "B55/PP2A substrate recruitment as defined by the retinoblastoma-related protein p107"  eLife 10, e63181-e63181 (2021).
PubMed: 34661528

Assembly members:

Assembly members:
p107, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTHMT

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts147
15N chemical shifts65
1H chemical shifts65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p1071

Entities:

Entity 1, p107 78 residues - Formula weight is not available

Residue 1-2 represent a cloning artifact. Residue 3-78 represent human p107 residue 612-687.

1   GLYHISMETPROMETSERPROLEUMETHIS
2   PROARGVALLYSGLUVALARGTHRASPSER
3   GLYSERLEUARGARGASPMETGLNPROLEU
4   SERPROILESERVALHISGLUARGTYRSER
5   SERPROTHRALAGLYSERALALYSARGARG
6   LEUPHEGLYGLUASPPROPROLYSGLUMET
7   LEUMETASPLYSILEILETHRGLUGLYTHR
8   LYSLEULYSILEALAPROSERSER

Samples:

sample_1: p107, [U-99% 13C; U-99% 15N], 0.7 mM; sodium chloride 50 mM; TCEP 0.5 mM; sodium phosphate 20 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 70 mM; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

cara v1.9.2, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Neo 600 MHz

Related Database Links:

UniprotKB P28749

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks