BMRB Entry 28089

Name: dUnr CSD6
Published: 2021-09-21

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28089

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

dUnr CSD6

Deposition date:

2020-02-18

Original release date:

2021-09-21

Authors:

Hollmann, Nele Merret; Hennig, Janosch

Citation:

Citation: Hollmann, Nele Merret; Jagtap, Pravin Kumar Ankush; Masiewicz, Pawel; Guitart, Tanit; Simon, Bernd; Provaznik, Jan; Stein, Frank; Haberkant, Per; Sweetapple, Lara Jayne; Villacorte, Laura; Mooijman, Dylan; Benes, Valdimir; Savitski, Mikhail; Gebauer, Fatima; Hennig, Janosch. "Pseudo-RNA binding domains mediate RNA structure specificity in Upstream of N-Ras" Cell Rep. 32, 107930-107930 (2020).
PubMed: 32697992

Assembly members:

Assembly members:
dUnr_CSD6, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dUnr_CSD6: GAFETLVASNIEGCVTREAP KSPIKSQDRVEGGVITYEHA DVKKTIMYFLKDCEKPPRIG ERVRFDIYMVKRNKECIAVN VQQVSLH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	155
15N chemical shifts	80
1H chemical shifts	80

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dUnr CSD6	1

Entities:

Entity 1, dUnr CSD6 87 residues - Formula weight is not available

The first two residues (GA) are leftovers from the restriction site.

1

GLY

ALA

PHE

GLU

THR

LEU

VAL

ALA

SER

ASN

2

ILE

GLU

GLY

CYS

VAL

THR

ARG

GLU

ALA

PRO

3

LYS

SER

PRO

ILE

LYS

SER

GLN

ASP

ARG

VAL

4

GLU

GLY

VAL

ILE

THR

TYR

GLU

HIS

ALA

5

ASP

VAL

LYS

THR

ILE

MET

TYR

PHE

LEU

6

LYS

ASP

CYS

GLU

LYS

PRO

ARG

ILE

GLY

7

GLU

ARG

VAL

ARG

PHE

ASP

ILE

TYR

MET

VAL

8

LYS

ARG

ASN

LYS

GLU

CYS

ILE

ALA

VAL

ASN

9

VAL

GLN

VAL

SER

LEU

HIS

Samples:

sample_1: dUnr CSD6, [U-99% 13C; U-99% 15N], 1.2 mM; sodium chloride 50 mM; sodium phosphate 20 mM; DTT 1 mM

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Cara, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance III 700 MHz

UNP	Q9VSK3
AlphaFold	Q9VSK3