BMRB Entry 28084

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28084

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Title:
1H, 13C, and 15N backbone assignments of the Brain And Acute Leukemia Cytoplasmic (BAALC) protein
Deposition date:
2020-02-18
Original release date:
2021-02-18
Authors:
Lang, Andras; Wiedemann, Christoph; Ohlenschlaeger, Oliver
Citation:

Citation: Lang, Andras; Kumar, Amit; Jirschitzka, Jan; Bordusa, Frank; Ohlenschlaeger, Oliver; Wiedemann, Christoph. "1H, 13C, and 15N backbone assignments of the Brain And Acute Leukemia Cytoplasmic (BAALC) protein"  Biomol. NMR Assignments 14, 163-168 (2020).
PubMed: 32240523

Assembly members:

Assembly members:
BAALC, polymer, 181 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BAALC: GMGCGGSRADAIEPRYYESW TRETESTWLTYTDSDAPPSA AAPDSGPEAGGLHSVLEAEK SKIKAPTDSVSDEGLFSASK MAPLAVFSHGMLEDGLPSNG VPRSTAPGGIPNPEKKTNCE TQCPNPQSLSSGPLTQKQNG LQTTEAKRDAKRMPAKEVTI NVTDSIQQMDRSRRITKNCV N

Data sets:
Data typeCount
13C chemical shifts540
15N chemical shifts163
1H chemical shifts354

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BAALC1

Entities:

Entity 1, BAALC 181 residues - Formula weight is not available

Residues 1 represents a non-native cloning artifact

1   GLYMETGLYCYSGLYGLYSERARGALAASP
2   ALAILEGLUPROARGTYRTYRGLUSERTRP
3   THRARGGLUTHRGLUSERTHRTRPLEUTHR
4   TYRTHRASPSERASPALAPROPROSERALA
5   ALAALAPROASPSERGLYPROGLUALAGLY
6   GLYLEUHISSERVALLEUGLUALAGLULYS
7   SERLYSILELYSALAPROTHRASPSERVAL
8   SERASPGLUGLYLEUPHESERALASERLYS
9   METALAPROLEUALAVALPHESERHISGLY
10   METLEUGLUASPGLYLEUPROSERASNGLY
11   VALPROARGSERTHRALAPROGLYGLYILE
12   PROASNPROGLULYSLYSTHRASNCYSGLU
13   THRGLNCYSPROASNPROGLNSERLEUSER
14   SERGLYPROLEUTHRGLNLYSGLNASNGLY
15   LEUGLNTHRTHRGLUALALYSARGASPALA
16   LYSARGMETPROALALYSGLUVALTHRILE
17   ASNVALTHRASPSERILEGLNGLNMETASP
18   ARGSERARGARGILETHRLYSASNCYSVAL
19   ASN

Samples:

sample_1: BAALC, [U-99% 13C; U-99% 15N], 1.55 mg/mL; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

UNP Q8WXS3
AlphaFold Q9HA93

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks