BMRB Entry 28065

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28065

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Title:
Backbone N, HN, CO and CA Chemical Shift Assignments for Tau (1-239)
Deposition date:
2020-01-27
Original release date:
2020-10-14
Authors:
Ibanez de Opakua, Alain; Zweckstetter, Markus; Cima-Omori, Maria Sol
Citation:

Citation: Ukmar-Godec, Tina; Fang, Pan; Ibanez de Opakua, Alain; Henneberg, Fabian; Godec, Aljaz; Pan, Kuan-Ting; Cima-Omori, Maria Sol; Chari, Ashwin; Mandelkow, Eckhard; Urlaub, Henning; Zweckstetter, Markus. "PProteasomal degradation of the intrinsically disordered protein tau at single-residue resolution"  Sci. Adv. 6, eaba3916-eaba3916 (2020).
PubMed: 32832664

Assembly members:

Assembly members:
Tau_(1-239), polymer, 239 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pNG2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tau_(1-239): MAEPRQEFEVMEDHAGTYGL GDRKDQGGYTMHQDQEGDTD AGLKESPLQTPTEDGSEEPG SETSDAKSTPTAEDVTAPLV DEGAPGKQAAAQPHTEIPEG TTAEEAGIGDTPSLEDEAAG HVTQARMVSKSKDGTGSDDK KAKGADGKTKIATPRGAAPP GQKGQANATRIPAKTPPAPK TPPSSGEPPKSGDRSGYSSP GSPGTPGSRSRTPSLPTPPT REPKKVAVVRTPPKSPSSA

Data sets:
Data typeCount
13C chemical shifts437
15N chemical shifts205
1H chemical shifts205

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tau (1-239)1

Entities:

Entity 1, Tau (1-239) 239 residues - Formula weight is not available

1   METALAGLUPROARGGLNGLUPHEGLUVAL
2   METGLUASPHISALAGLYTHRTYRGLYLEU
3   GLYASPARGLYSASPGLNGLYGLYTYRTHR
4   METHISGLNASPGLNGLUGLYASPTHRASP
5   ALAGLYLEULYSGLUSERPROLEUGLNTHR
6   PROTHRGLUASPGLYSERGLUGLUPROGLY
7   SERGLUTHRSERASPALALYSSERTHRPRO
8   THRALAGLUASPVALTHRALAPROLEUVAL
9   ASPGLUGLYALAPROGLYLYSGLNALAALA
10   ALAGLNPROHISTHRGLUILEPROGLUGLY
11   THRTHRALAGLUGLUALAGLYILEGLYASP
12   THRPROSERLEUGLUASPGLUALAALAGLY
13   HISVALTHRGLNALAARGMETVALSERLYS
14   SERLYSASPGLYTHRGLYSERASPASPLYS
15   LYSALALYSGLYALAASPGLYLYSTHRLYS
16   ILEALATHRPROARGGLYALAALAPROPRO
17   GLYGLNLYSGLYGLNALAASNALATHRARG
18   ILEPROALALYSTHRPROPROALAPROLYS
19   THRPROPROSERSERGLYGLUPROPROLYS
20   SERGLYASPARGSERGLYTYRSERSERPRO
21   GLYSERPROGLYTHRPROGLYSERARGSER
22   ARGTHRPROSERLEUPROTHRPROPROTHR
23   ARGGLUPROLYSLYSVALALAVALVALARG
24   THRPROPROLYSSERPROSERSERALA

Samples:

sample_1: Tau (1-239), [U-99% 13C; U-99% 15N], 125 uM; sodium phosphate 50 mM; sodium azide 0.01%; D2O 5%; DSS 50 uM

sample_conditions_1: ionic strength: 0.154 M; pH: 6.8; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks