BMRB Entry 28058

Title:
NMR assignment of self-processing module of FrpC protein of Neisseria meningitidis loaded with calcium ions
Deposition date:
2020-01-02
Original release date:
2020-02-20
Authors:
Kuban, Vojtech; Zidek, Lukas; Macek, Pavel; Hritz, Jozef; Bumba, Ladislav; M., Faldyna; K., Nedbalcova; K., Nechvatalova
Citation:

Citation: Kuban, V.; Macek, P.; Hritz, J.; Nechvatalova, K.; Nedbalcova, K.; Faldyna, M.; Zidek, L.; Bumba, L.. "Structural basis of Ca 2+ -dependent self-processing activity of repeat-in-toxin proteins"  mBio 11, e00226-20-e00226-20 (2020).
PubMed: 32184239

Assembly members:

Assembly members:
SPM, polymer, 179 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Neisseria meningitidis   Taxonomy ID: 487   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Neisseria meningitidis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSPM-His

Data typeCount
13C chemical shifts608
15N chemical shifts162
1H chemical shifts974
T1 relaxation values124
T2 relaxation values124
heteronuclear NOE values124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SPM1
2CA2

Entities:

Entity 1, SPM 179 residues - Formula weight is not available

1   PROLEUALALEUASPLEUASPGLYASPGLY
2   ILEGLUTHRVALALATHRLYSGLYPHESER
3   GLYSERLEUPHEASPHISASNARGASPGLY
4   ILEARGTHRALATHRGLYTRPVALSERALA
5   ASPASPGLYLEULEUVALARGASPLEUASN
6   GLYASNGLYILEILEASPASNGLYALAGLU
7   LEUPHEGLYASPASNTHRLYSLEUALAASP
8   GLYSERPHEALALYSHISGLYTYRALAALA
9   LEUALAGLULEUASPSERASNGLYASPASN
10   ILEILEASNALAALAASPALAALAPHEGLN
11   SERLEUARGVALTRPGLNASPLEUASNGLN
12   ASPGLYILESERGLNALAASNGLULEUARG
13   THRLEUGLUGLULEUGLYILEGLNSERLEU
14   ASPLEUALATYRLYSASPVALASNLYSASN
15   LEUGLYASNGLYASNTHRLEUALAGLNGLN
16   GLYSERTYRTHRLYSTHRASNGLYTHRTHR
17   ALALYSMETGLYASPLEULEULEUALAALA
18   ASPASNLEUHISSERARGPHELEUGLU

Entity 2, CA - Ca - 40.078 Da.

1   CA

Samples:

sample_1: SPM, [U-100% 13C; U-100% 15N], 0.4 mM; TRIS 5 mM; sodium chloride 50 mM; sodium azide 0.1%; Calcium chloride 10 mM

sample_conditions_1: ionic strength: 65 mM; pH: 7.4; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
2D R2 15N,1H correlationsample_1isotropicsample_conditions_1
2D R1 15N,1H correlationsample_1isotropicsample_conditions_1
2D NOE 15N,1H correlationsample_1isotropicsample_conditions_1

Software:

SPARKY v3.115, Goddard - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN v3.2, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 850 MHz
  • Bruker Avance 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks