BMRB Entry 28054

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28054

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Title:
ILVM methyl assignments for Pyrococcus furiosus Mre11 nuclease and capping domain
Deposition date:
2019-12-16
Original release date:
2019-12-20
Authors:
Beikzadeh, Mahtab; Kaur, Navneet; Latham, Michael
Citation:

Citation: Rahman, Samiur; Beikzadeh, Mahtab; Canny, Marella; Kaur, Navneet; Latham, Michael. "Mutation of Conserved Mre11 Residues Alter Protein Dynamics to Separate Nuclease Functions"  J. Mol. Biol. 432, 3289-3308 (2020).
PubMed: 32246962

Assembly members:

Assembly members:
Mre11_ND, polymer, 333 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 29

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Mre11_ND: MKFAHLADIHLGYEQFHKPQ REEEFAEAFKNALEIAVQEN VDFILIAGDLFHSSRPSPGT LKKAIALLQIPKEHSIPVFA IEGNHDRTQRGPSVLNLLED FGLVYVIGMRKEKVENEYLT SERLGNGEYLVKGVYKDLEI HGMKYMSSAWFEANKEILKR LFRPTDNAILMLHQGVREVS EARGEDYFEIGLGDLPEGYL YYALGHIHKRYETSYSGSPV VYPGSLERWDFGDYEVRYEW DGIKFKERYGVNKGFYIVED FKPRFVEIKVRPFIDVKIKG SEEEIRKAIKRLIPLIPKNA YVRLNIGWRKPFDLTEIKEL LNVEYLKIDTWRI

Data sets:
Data typeCount
13C chemical shifts134
1H chemical shifts402

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Mre11 ND1

Entities:

Entity 1, Mre11 ND 333 residues - Formula weight is not available

1   METLYSPHEALAHISLEUALAASPILEHIS
2   LEUGLYTYRGLUGLNPHEHISLYSPROGLN
3   ARGGLUGLUGLUPHEALAGLUALAPHELYS
4   ASNALALEUGLUILEALAVALGLNGLUASN
5   VALASPPHEILELEUILEALAGLYASPLEU
6   PHEHISSERSERARGPROSERPROGLYTHR
7   LEULYSLYSALAILEALALEULEUGLNILE
8   PROLYSGLUHISSERILEPROVALPHEALA
9   ILEGLUGLYASNHISASPARGTHRGLNARG
10   GLYPROSERVALLEUASNLEULEUGLUASP
11   PHEGLYLEUVALTYRVALILEGLYMETARG
12   LYSGLULYSVALGLUASNGLUTYRLEUTHR
13   SERGLUARGLEUGLYASNGLYGLUTYRLEU
14   VALLYSGLYVALTYRLYSASPLEUGLUILE
15   HISGLYMETLYSTYRMETSERSERALATRP
16   PHEGLUALAASNLYSGLUILELEULYSARG
17   LEUPHEARGPROTHRASPASNALAILELEU
18   METLEUHISGLNGLYVALARGGLUVALSER
19   GLUALAARGGLYGLUASPTYRPHEGLUILE
20   GLYLEUGLYASPLEUPROGLUGLYTYRLEU
21   TYRTYRALALEUGLYHISILEHISLYSARG
22   TYRGLUTHRSERTYRSERGLYSERPROVAL
23   VALTYRPROGLYSERLEUGLUARGTRPASP
24   PHEGLYASPTYRGLUVALARGTYRGLUTRP
25   ASPGLYILELYSPHELYSGLUARGTYRGLY
26   VALASNLYSGLYPHETYRILEVALGLUASP
27   PHELYSPROARGPHEVALGLUILELYSVAL
28   ARGPROPHEILEASPVALLYSILELYSGLY
29   SERGLUGLUGLUILEARGLYSALAILELYS
30   ARGLEUILEPROLEUILEPROLYSASNALA
31   TYRVALARGLEUASNILEGLYTRPARGLYS
32   PROPHEASPLEUTHRGLUILELYSGLULEU
33   LEUASNVALGLUTYRLEULYSILEASPTHR
34   TRPARGILE

Samples:

sample_1: Mre11 ND, [U-98% 2H; U-100% 12C; Iled1 13CH3; Leud 13CH3/12CD3; Valg 13CH3/12CD3; Mete 13CH3], 0.5 mM; sodium sulfate 300 mM; HEPES 25 mM; EDTA 0.5 mM

sample_conditions_1: ionic strength: 300 mM; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2H 12C, methyl-TROSY HMQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - processing

NMR spectrometers:

  • Agilent DD2 600 MHz