BMRB Entry 28047

Name: RalA.GDP
Published: 2020-02-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28047

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RalA.GDP

Deposition date:

2019-11-25

Original release date:

2020-02-26

Authors:

Shafiq, Arooj; Owen, Darerca; Mott, Helen

Citation:

Citation: Shafiq, Arooj; Campbell, Louise; Owen, Darerca; Mott, Helen. "NMR resonance assignments for the active and inactive conformations of the small G protein RalA" Biomol. NMR Assignments 14, 87-91 (2020).
PubMed: 31916136

Assembly members:

Assembly members:
RalA, polymer, 184 residues, Formula weight is not available
GUANOSINE-5'-DIPHOSPHATE, non-polymer, 443.201 Da.

Natural source:

Natural source: Common Name: cotton-top tamarin Taxonomy ID: 9490 Superkingdom: Eukaryota Kingdom: metazoa Genus/species: Saguinus Oedipus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGex6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RalA: MAANKPKGQNSLALHKVIMV GSGGVGKSALTLQFMYDEFV EDYEPTKADSYRKKVVLDGE EVQIDILDTAGQEDYAAIRD NYFRSGEGFLCVFSITEMES FAATADFREQILRVKEDENV PFLLVGNKSDLEDKRQVSVE EAKNRADQWNVNYVETSAKT RANVDKVFFDLMREIRARKM EDSK

Data sets:

assigned_chemical_shifts
- Protein
- GDP

Data type	Count
15N chemical shifts	171
1H chemical shifts	708

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RalA	1
2	GDP	2

Entities:

Entity 1, RalA 184 residues - Formula weight is not available

1

MET

ALA

ASN

LYS

PRO

LYS

GLY

GLN

ASN

2

SER

LEU

ALA

LEU

HIS

LYS

VAL

ILE

MET

VAL

3

GLY

SER

GLY

VAL

GLY

LYS

SER

ALA

LEU

4

THR

LEU

GLN

PHE

MET

TYR

ASP

GLU

PHE

VAL

5

GLU

ASP

TYR

GLU

PRO

THR

LYS

ALA

ASP

SER

6

TYR

ARG

LYS

VAL

LEU

ASP

GLY

GLU

7

GLU

VAL

GLN

ILE

ASP

ILE

LEU

ASP

THR

ALA

8

GLY

GLN

GLU

ASP

TYR

ALA

ILE

ARG

ASP

9

ASN

TYR

PHE

ARG

SER

GLY

GLU

GLY

PHE

LEU

10

CYS

VAL

PHE

SER

ILE

THR

GLU

MET

GLU

SER

11

PHE

ALA

THR

ALA

ASP

PHE

ARG

GLU

GLN

12

ILE

LEU

ARG

VAL

LYS

GLU

ASP

GLU

ASN

VAL

13

PRO

PHE

LEU

VAL

GLY

ASN

LYS

SER

ASP

14

LEU

GLU

ASP

LYS

ARG

GLN

VAL

SER

VAL

GLU

15

GLU

ALA

LYS

ASN

ARG

ALA

ASP

GLN

TRP

ASN

16

VAL

ASN

TYR

VAL

GLU

THR

SER

ALA

LYS

THR

17

ARG

ALA

ASN

VAL

ASP

LYS

VAL

PHE

ASP

18

LEU

MET

ARG

GLU

ILE

ARG

ALA

ARG

LYS

MET

19

GLU

ASP

SER

LYS

Entity 2, GDP - 443.201 Da.

1

GDP

Samples:

sample_1: RalA, [U-15N], 0.75 ± 0.1 mM; GDP 0.75 ± 0.1 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CCPN, CCPN - chemical shift assignment

NMR spectrometers:

Bruker DRX 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks